| 产2，3-丁二醇的产气肠杆菌株的诱变和筛选 |
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| 引用本文： | 赵世敏,严群,阮文权,邹华. 产2，3-丁二醇的产气肠杆菌株的诱变和筛选[J]. 食品与发酵工业, 2008, 34(3): 25-29 |
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| 作者姓名： | 赵世敏 严群 阮文权 邹华 |
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| 作者单位： | 1. 江南大学工业生物技术教育部重点实验室,江苏无锡,214122
2. 江南大学工业生物技术教育部重点实验室,江苏无锡,214122;江南大学生物工程学院,江苏无锡,214122 |
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| 基金项目： | 江苏省高技术研究发展计划项目 , 江苏省自然科学基金 |
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| 摘 要： | 以产气肠杆菌为原始菌株,采用紫外线、亚硝基胍复合诱变进行诱变育种,以产酸圈和产物耐受相结合的筛选方法,获得了1株高产2,3-丁二醇的优良突变菌株,其产量达到24.20 g/L,比原始菌株(6.28 g/L)提高了3.8倍,且遗传性质稳定。该诱变方法和筛选方法目标明确,易操作,对于其他具有工业应用价值菌株的筛选有一定的借鉴意义。
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| 关 键 词： | 产气肠杆菌 2 3-丁二醇 诱变育种 |
| 修稿时间： | 2007-10-17 |
Mutation and Screening of Enterobacter aerogenes for the Production of 2,3-butanediol |
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| Zhao Shimin,Yan Qun,Ruan Wenquan,Zou Hua. Mutation and Screening of Enterobacter aerogenes for the Production of 2,3-butanediol[J]. Food and Fermentation Industries, 2008, 34(3): 25-29 |
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| Authors: | Zhao Shimin Yan Qun Ruan Wenquan Zou Hua |
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| Abstract: | The combinational mutation of ultraviolet and nitrosoguanidine on Enterobacter aerogenes was conducted,and the fast screening method based on acid production and 2,3-butanediol tolerance were estab- lished.After mutation,the 2,3—butanediol production ability was increased to 24.20 g/L,which was 3.8 times than that of the wild type.Moreover,heritage stability was confirmed after several generations of sub- clonation and physiological and biochemical tests. |
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| Keywords: | Enterobacter aerogenes 2,3-butanediol mutation breeding |
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