| 免疫亲和柱——高效液相色谱法测定肾脏中的赭曲霉毒素A |
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| 引用本文： | 韩惠雯,黄菲菲. 免疫亲和柱——高效液相色谱法测定肾脏中的赭曲霉毒素A[J]. 中国食品卫生杂志, 2009, 21(3) |
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| 作者姓名： | 韩惠雯 黄菲菲 |
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| 作者单位： | 农业部食品质量监督检验测试中心,上海,200072 |
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| 摘 要： | 目的针对动物肾脏中可能污染的赭曲霉毒素A(OTA),建立了肾脏中赭曲霉毒素A的高效液相色谱方法。方法试样用磷酸酸化后经乙酸乙酯提取,免疫亲和柱净化,以乙腈-水-冰醋酸(450+525+25)为流动相,C18柱分离并通过荧光检测器定量。结果赭曲霉毒素A标准溶液浓度在0.10～20μg/L范围内呈线性相关(r=1.000 0),不同浓度水平的添加回收率为72.5%～87.4%,检出限为0.012μg/kg。结论使用免疫亲和柱净化能够达到良好的净化效果,是一种准确、方便的测定猪肾中赭曲霉毒素A的分析方法。
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| 关 键 词： | 食品污染 真菌毒素 化学 分析 色谱法 亲和 高压液相 肾 |
Determination of Ochratoxin A in Kidney by Immunoaffinity Column and High Performance Liquid Chromatography |
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| HAN Hui-wen,HUANG Fei-fei. Determination of Ochratoxin A in Kidney by Immunoaffinity Column and High Performance Liquid Chromatography[J]. Chinese Journal of Food Hygiene, 2009, 21(3) |
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| Authors: | HAN Hui-wen HUANG Fei-fei |
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| Affiliation: | Shanghai Food Quality Supervision and Inspection Center of Ministry of Agriculture of China;Shanghai 200072;China |
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| Abstract: | Objective To develop a method on Ochratoxin A(OTA)in kidney determinate by immuno-affinity column(IAC) and high performance liquid chromatography(HPLC).Method Samples were extracted by ethyl acetate,after cleaned up by IAC.The contents of OTA were quantified by reverse-HPLC with fluorometric detector,and acetonitrile-water-acetic acid(450+525+25) were used as mobile phase.Results The method had a good relativity(r=1.000?0) and good analytic precision(RSD<10%).The recoveries of the method were within the sco... |
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| Keywords: | Food Contamination Mycotoxins Chemistry Analytical Chromatography Affinity High Pressure Liquid Kidney |
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