头孢唑啉钠在吐温80-硫酸钠液-固萃取体系中的分配行为

对头孢菌素类抗生素--头孢唑啉钠在吐温80-硫酸钠液-固萃取体系中的分配行为做了初步研究.试验了成相聚合物吐温80的体积分数、成相盐硫酸钠的浓度和体系pH值对头孢唑啉钠在吐温80固相中正萃取的影响;硫酸钠浓度对头孢唑啉钠在硫酸钠盐水相中反萃取的影响.结果表明:吐温80在体系中体积分数为12％,硫酸钠浓度为1.48 mol／L,pH 5.0时,吐温80固相对头孢唑啉钠的正萃取率为72.0％;正萃取后,收集并溶解吐温80固相,调节二次成相硫酸钠浓度为0.53 mol／L,pH 9.0,硫酸钠盐水相对头孢唑啉钠的反萃取率为70 %.结果表明,头孢唑林钠在该体系中有较高的萃取率和较好的稳定性.

Primary Study on the Partition of Cephalosrins in Tween80-Na2SO4 Liquid-Solid Extraction System

The distribution of small biomolecular, cefrazolin sodium, in the polymer-salt aqueous liquid-solid extraction system, Tween80-Na2SO4 system, was investigated. Some parameters contributed to the partitioning behavior of cephalosporins, such as the concentration of the phase-forming polymer, Tween80, the phase-forming slat, Na2 SO4 and the pH of the system were studied. The optimal conditions were obtained. Under the optimal condition, where the concentration of Tween80 was 12% (volume fraction), Na2 SO4 was 1. 48 mol/L, and pH 5. 0, the recovery of cefrazolin sodium in Tween80 phase was 73%. With the concentration of phase-forming salt,Na2 SO4, adjusted to 0. 53 mol/L at pH 9. 0,the dissolved Tween80 reformed the liquid-solid two phase system, and the cefrazolin sodium was extracted to the salt aqueous with the recovery near 70%. From the result, it is concluded that, the polymer-salt liquid-solid extraction system could be applied for the partitioning of small biomoleculars such as cepholosporins with satisfied recovery and imperceptible damages to the bioactivities of the biomoleculars.