| 荧光素-环胺-Cu(Ⅱ)离子荧光探针的合成及其在一氧化氮检测中的应用 |
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| 引用本文： | 陈云龙,王明慧.荧光素-环胺-Cu(Ⅱ)离子荧光探针的合成及其在一氧化氮检测中的应用[J].青岛科技大学学报,2012(4):372-376. |
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| 作者姓名： | 陈云龙 王明慧 |
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| 作者单位： | 青岛科技大学化学与分子工程学院 |
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| 基金项目： | 山东省自然科学基金项目(ZR2009BM019) |
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| 摘 要： | 合成了一种新型一氧化氮分子荧光探针并对其结构进行了表征,此荧光探针为连接有1,4,8,11-四氮杂环十四烷环胺化合物的荧光素-环胺-Cu(Ⅱ)离子配合物,其本身无荧光,当与NO反应后,在λex=465nm光激发下产生荧光且最大发射波长λem=524nm。体系的pH值对荧光强度有影响,在pH为4.5时荧光强度最大。在H2O2,ONOO-存在下,荧光探针对NO表现出很好的选择性。以NOC-18为NO释放剂,在NOC-18浓度为4×10-5～14×10-5 mol.L-1范围内建立了NOC-18浓度和荧光强度间的关系曲线,荧光强度随NOC-18浓度的增加而增大,可实现对NO的直接检测。
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| 关 键 词： | 荧光探针 一氧化氮 荧光素-环胺-Cu(Ⅱ)离子配合物 1 4 8 11-四氮杂环十四烷环胺 |
Synthesis of Fluorescein-based Copper Fluorescent Probe and Its Application in NO Detection |
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| CHEN Yun-long,WANG Ming-hui.Synthesis of Fluorescein-based Copper Fluorescent Probe and Its Application in NO Detection[J].Journal of Qingdao University of Science and Technology:Natutral Science Edition,2012(4):372-376. |
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| Authors: | CHEN Yun-long WANG Ming-hui |
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| Affiliation: | (College of Chemistry and Molecular Engineering,Qingdao University of Science and Technology,Qingdao 266042,China) |
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| Abstract: | A novel fluorescent probe for NO,fluorescein-Cu(Ⅱ) complex connecting with 1,4,8,11-tetraazacyclotetra-decane,was synthesized and characterized.The complex himself showed non-fluorescent,when reacted with NO,it showed a strong fluorescent peak at λem =524 nm and λex=465 nm.The pH could affect fluorescence intensity and there was a maximum value when pH=4.5.The fluorescent probe exhibited an excellent selectivity to NO in the presence of H2O2 or ONOO-.A relationship curve was developed between fluorescent intensity and concentration of NOC-18(a donor of NO).At range of 4×10-5 to 14×10-5 mol·L-1 of NOC-18,the fluorescent intensity was increased with increasing of NOC-18 concentration.Therefore,NO can be determined directly through measuring fluorescent intensity. |
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| Keywords: | fluorescent probe nitrogen monoxide fluorescein-Cu(II) complex 1 4 8 11-tetraaza-cyclotetradecane |
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