气相色谱法检测饮料中二羰基化合物

建立同时在线检测丙酮醛和乙二醛的气相色谱方法。确定检测二羰基化合物的最佳条件为：以丁二酮为内标，以邻苯二胺为衍生化试剂，邻苯二胺的用量67 倍于二羰基化合物、衍生化时间10 min、萃取溶剂二氯甲烷、超声时间15 min、萃取2 次、柱箱初始温度40 ℃、程序升温5 ℃/min，色谱柱载气流量2.0 mL/min，分流比1∶1。丙酮醛和乙二醛的定量限（RSN≈10）分别为0.06 mg/L和0.08 mg/L，检出限（RSN≈ 3）分别为0.02 mg/L和0.03 mg/L，方法灵敏度高。

Detection of Dicarbonyl Compounds in Beverages by Gas Chromatography

This study established an efficient method for the detection of methylglyoxal (MGO) and glyoxal (GO) with a gas
chromatograph (GC). The optimum experimental conditions were set as follows: ratio of O-phenylenediamine to α-dicarbonyl
compounds, 67; derivatization time, 10 min; extraction solvent, methylene chloride; ultrasonic extraction time, 15 min; and
the number of extraction, 2. The injector was operated in split mode with a split ratio of 1:1, and the carrier gas (helium)
flow rate through the chromatographic column was 2.0 mL/min. The GC oven temperature was programmed as follows: the
initial oven temperature was set as 40 ℃ and then increased at a rate of 5 ℃/min. The minimum quantitation limits (RSN
approximately equal to 10) and detection limits (RSN approximately equal to 3) for MGO and GO were 0.06 and 0.08 mg/L,
and 0.02 and 0.03 mg/L, respectively. This method was sensitive and efficient.