石榴花精油成分分析及清除自由基能力评价

采用气相色谱-质谱联用技术分析石榴花精油的化学成分，通过对2,2-联氮基-双-（3-乙基苯并噻唑啉-6-磺酸）二氨盐（2,2’-amino-di (2-ethyl -benzothiazoline sulphonic acid-6)ammonium salt，ABTS）及1,1-二苯基-2-三硝基苯肼（1,1 dipheny1-2 picryl-hydrazyl，DPPH）自由基的清除作用评价其抗氧化能力。结果表明，从石榴花精油中共分离出52 个质谱峰，鉴定出32 种成分，其中有9 种烷烃类化合物（其中有5 种环烷烃和4 种直链烷烃）、7 种萜烯类化合物、6 种酯类化合物、4 种芳香族醇类化合物、酮类和醛类化合物各2 种、生物碱类和磺酸盐类化合物各1 种。对ABTS＋·和DPPH自由基清除力分别为0.029 mg/mg VC当量和0.01 mg/mg VC当量。表明石榴花精油中含有多种有应用价值的化学成分，是一种具有开发利用价值的天然资源。

Analysis of the Chemical Constituents of Essential Oil from Pomegranate Flower and Evaluation of Its Free Radical Scavenging Ability

Gas chromatography-mass spectrometer (GC-MS) was used to analyze the chemical components in essential oil
of pomegranate flower. The free radical scavenging ability of the oil was assessed by using 1,1 dipheny1-2 picryl-hydrazyl
(DPPH) system and 2,2’-amino-di (2-ethyl -benzothiazoline sulphonic acid-6) ammonium salt (ABTS+·) system. The
results showed that 52 peaks were detected from the pomegranate flower oil and 32 compounds were identified, including
9 alkanes (5 naphthenic hydrocarbons and 4 straight-chain alkanes), 7 terpenes, 6 esters, 4 alcohols, 2 ketones and aldehydes,
1 alkaloid and sulfonate. The free radical scavenging capacity of the oil against ABTS+· and DPPH free radicals were 0.029 and
0.01 mg/mg vitamin C (VC) equivalent, respectively. These results indicate that pomegranate flower essential oil contains a
variety of chemical constituents with potential applications as a valuable natural resource.