| 志贺氏菌标准物质的制备方法 |
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| 引用本文： | 柯 璐,戴晓丽,林 杰,黄嫦娇,曾维扬,张温玲.志贺氏菌标准物质的制备方法[J].食品科学,2015,36(24):253-259. |
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| 作者姓名： | 柯 璐 戴晓丽 林 杰 黄嫦娇 曾维扬 张温玲 |
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| 作者单位： | 福建出入境检验检疫局技术中心，福建省检验检疫技术研究重点实验室，福建 福州 350003 |
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| 基金项目： | 福建出入境检验检疫局科技计划项目（FK2013-22） |
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| 摘 要： | 采用冷冻干燥法制备标准物质。通过正交试验法选择冻干保护率最高的保护剂配方方案。以鸡肉粉作为标准物质基质，按比例与保护剂配方混合，添加拟定菌数的新鲜菌液后进行冷冻干燥。实验制备的标准物质样品其特性值（含菌数）为530 CFU/瓶，不确定度为24 CFU/瓶。冷冻干燥样品在－20 ℃及4 ℃条件下保存12 个月，特性值稳定；25 ℃条件下保存9 个月，特性值稳定；60 ℃条件下保存，稳定性差。
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| 关 键 词： | 宋氏志贺氏菌 标准物质 基质 鸡肉 |
Preparation of Reference Materials for Shigella |
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| KE Lu,DAI Xiaoli,LIN Jie,HUANG Changjiao,ZENG Weiyang,ZHANG Wenling.Preparation of Reference Materials for Shigella[J].Food Science,2015,36(24):253-259. |
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| Authors: | KE Lu DAI Xiaoli LIN Jie HUANG Changjiao ZENG Weiyang ZHANG Wenling |
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| Affiliation: | Fujian Provincial Key Laboratory of Inspection and Quarantine Technology Research, Center of Technology,
Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350003, China |
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| Abstract: | This study aimed to prepare reference materials for Shigella by a freeze-drying method. Orthogonal array design
was applied to choose the optimal formulation of lyoprotectant that provided maximum protection efficiency. Chicken powder was
used as the substrate for reference materials, which was mixed proportionally with the lyoprotectant and freeze-dried after addition
of the fresh bacterial solution with a predetermined number of cells. The reference materials had a characteristic value (bacterial
count) of 530 CFU/bottle with an uncertainty of 24 CFU/bottle. At ?20 and 4 ℃ the freeze-dried samples could be stored for 12
months with good stability of the characteristic value. At 25 ℃,the storage life of the freeze-dried samples was 9 months, during
which the characteristic value remained stable. However, at 60 ℃, the stability was poor. |
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| Keywords: | Shigella sonnei reference materials matrix chicken |
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