The VIT technology for rapid detection of Listeria monocytogenes and other Listeria spp |
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Authors: | Stephan Roger Schumacher Sandra Zychowska Marzena Anna |
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Affiliation: | Institute for Food Safety and Hygiene, Faculty of Veterinary Medicine, University of Zurich, Winterthurerstr. 272, CH-8057 Zurich, Switzerland. stephanr@fsafety.unizh.ch |
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Abstract: | Detection of Listeria monocytogenes is generally performed in a two-step cultural enrichment process and takes on average 1 week until the biochemical identification of a L. monocytogenes suspicious colony is completed. However, food processing companies depend increasingly on test methods, which attempt to generate results comparable to standard methods but in reduced time-frame and which allow to release produced batches dependent on such results. In the present study, the vermicon identification technology (VIT), a rapid commercial test system using fluorescently labelled gene probes, was compared to a cultural standard method. In total, 298 naturally contaminated samples were analysed. The sensitivity and the specificity of the VIT system were 100% for the detection of L. monocytogenes and 97.1% and 100%, respectively, for the detection of the genus Listeria. |
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