人尿酸氧化酶结构的同源模建和生物信息学分析

以微生物来源的尿酸氧化酶晶体结构为模板,根据推测的人尿酸氧化酶序列,利用同源模建技术预测人尿酸氧化酶的三维结构。人尿酸氧化酶结构具有两个相同的T-fold结构域。叠合不同来源的尿酸氧化酶结构证实,人尿酸氧化酶与其他来源尿酸氧化酶的活性中心的结构高度保守。另外找到一些不利于人尿酸氧化酶聚合的因素,这些因素可能是使人尿酸氧化酶基因在进化中失活的部分原因。

Homology modeling and bioinformatics analysis of three-dimensional structure of human urate oxidase

Based on it's deduced coding sequence, the tertiary structure of human urate oxidase was predicted by homology modeling technique using the crystal structures of urate oxidases from Aspergillus flavus and Arthrobacter globiformis as templates. The built model consists of two structurally equivalent T-fold domains. By superposition of the structures of urate oxidases from different organisms, it can be found that human urate oxidase displays strong homology in active site with other urate oxidase. Some residues with bad effect for human urate oxidase to form dimer and tetramer are examined. Those residues may be the partial cause for human urate oxidase gene became inactive during evolution.