植物油中角鲨烯的GC/MS分析

建立植物油中角鲨烯的GC/MS分析方法,植物油经KOH-甲醇法皂化和三氟化硼衍生,经HP-5MS毛细管柱分离,以二十四烷酸为内标,用GC/MS选择性离子监测对角鲨烯进行定量测定。方法在0.0625～2.00μg/mL范围内线性关系良好,相关系数r>0.9998;最低检测限为7.8ng/mL。日内精密度为1.63%~2.89%,日间精密度为1.70%~3.22%。加标平均回收率为89.58%～94.12%。用所建立的方法测定6种植物油中角鲨烯含量。

Determination of squalene in vegetable oils by GC/MS

A method was developed for the determination of squalene in vegetable oils by gas chromatog-raphy-mass spectrometry(GC/MS) with selective ion monitoring. After saponification with potassium hy-droxide and deveriation using borontriifluoride, vegetable oils were separated on a HP-5MS column. Ligno-ceric acid(CH3(CH2)22COOH) was selected as the internal standard. The The linear range of the method was 0.0625~2.00μg/mL (r=0.9998). The detection limit of squalene was 7.8ng/mL. The within-day RSD were 1.63%~2.89% The between-day RSD were 1.70%~3.22%, The average recovery of squalene was 90.60%. The method has been successfully applied in the determination of squalene in 6 vegetable oils.