Effects of urea infusion on the uterine luminal environment of dairy cows

Previous research indicates that high plasma urea nitrogen (PUN) concentrations are associated with decreased fertility in lactating dairy cows. The objective of this study was to monitor changes in the uterine environment during acute elevation of PUN. Lactating dairy cows (n = 8) were infused with saline or urea (0.01 g of urea/h per kg of body weight) through jugular vein catheters on d 7 after estrus. After 24 h, cows were switched to the opposite treatment for a second 24-h infusion period. Blood samples were collected every 2 h, and the pH within the lumen of the uterine horn ipsilateral to the corpus luteum was recorded every 6 h. At the end of each 24-h infusion period, 30 mL of sterile saline was flushed into the uterine lumen and immediately retrieved. Mean PUN concentration increased from 16.6 +/- 1.3 mg/dL during saline infusion to 22.6 +/- 1.3 mg/dL during urea infusion. Uterine pH decreased during urea infusion from 7.08 +/- 0.07 at 6 h to 6.88 +/- 0.08 at 18 h, but was unchanged during saline infusion (7.01 +/- 0.08 at 6 h to 7.06 +/- 0.07 at 18 h). Protein concentration, PGF(2alpha), and prostaglandin E(2) concentrations in uterine lavage samples were not different between treatments. The results of this study indicate that a short-term increase in PUN can exert direct effects on the uterine environment by decreasing uterine pH.