Iodine number determination of milk fat and vegetable fats by refractometry |
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Authors: | M K Rama Murthy G S Bhat |
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Affiliation: | (1) Dairy Chemistry Department, Southern Regional Station of National Dairy Research Institute, Hosur Road, 5600 30 Bangalore, India |
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Abstract: | A refractometric method for the estimation of iodine number of milk fat has been suggested. About 0.2 ml of milk fat was iodinated
with ca. 10 ml Wijs iodine reagent for 3 min using mercuric acetate as catalyst. The iodinated fat showed a higher refractive
index than the original fat. The changes in refractive indices showed a very high correlation with the iodine values of the
fats (T=0.9993). The average of the ratios of change in refractive index to iodine number was 50.7×10−5, from which the iodine number of milk fat can be calculated. The method can also be applied to vegetable fats. The ratios
of change in refractive index to iodine number for the oils of peanut, rapeseed, soybean, niger, sesame, and sunflower were
similar, and the average was 45.2×10−5. The ratio for linseed oil was 38.4×10−5, and for coconut fat it was similar to that of milk fat. |
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