| 极耐热内切葡聚糖酶Cel12B的高效表达 |
| |
| 引用本文： | 李相前,邵蔚蓝. 极耐热内切葡聚糖酶Cel12B的高效表达[J]. 食品与发酵工业, 2006, 32(2): 1-3 |
| |
| 作者姓名： | 李相前 邵蔚蓝 |
| |
| 作者单位： | 1. 江南大学工业生物技术教育部重点实验室,无锡,214036;准阴工学院,淮安,223001
2. 江南大学工业生物技术教育部重点实验室,无锡,214036;南京师范大学生命科学院,南京,210097 |
| |
| 基金项目： | 江苏省教育厅自然科学基金 |
| |
| 摘 要： | 海栖热袍菌 Thermotoga maritima 是嗜极端高温的厌氧细菌,其产生的极耐热葡聚糖酶具有很好的热稳定性和潜在的可观工业用途。但嗜高温菌基因在大肠杆菌中表达水平较低,T．m 内切葡聚糖酶 Cel12B 基因带有信号肽,应用在线预测分析,通过 PCR 方法分别克隆 Cel12B 完整基因和不带信号肽基因至 pET-20b 载体, 构建重组质粒 pET-20b-Cel12B 和 pET-20b-Cel12B-WS,转化至大肠杆菌 JM109DE3诱导表达后,分析酶活,结果表明去除信号肽重组菌单位酶活是未去除信号肽重组菌11倍多。
|
| 关 键 词： | 内切葡聚糖酶 海栖热袍菌 信号肽 表达 |
| 修稿时间： | 2005-10-31 |
High-level Expression of Endoglucans Cel12B Gene from Thermotago maritima in Escherichia coli |
| |
| Li Xiangqian,Shao Weilan. High-level Expression of Endoglucans Cel12B Gene from Thermotago maritima in Escherichia coli[J]. Food and Fermentation Industries, 2006, 32(2): 1-3 |
| |
| Authors: | Li Xiangqian Shao Weilan |
| |
| Abstract: | Thermotaga maritima is strictly anaerobic and extremely thermophilic bacteria.The endoglucanase found in T.maritirna showed extremely high thermostability and considerable potential in industrial applica- tion.But expression of Thermotaga gene in E coli is difficult.There is signal peptide in gene of endoglucanase Cel12B from T.maritima.The gene of endoglucanase Cel12B(with and without signal peptide sequence accord- ing to DNA analysis programs)was amplified by PCR and inserted into the plasmid pET-20b.The recombinant plasmid pET-20b-Cel12B and pET-20b-Cel12B-WS were transformed to E.coli JM109DE3,induced by IPTG and their gene expression levels were determined.The results showed that enzyme activity produced by pET-20b- Cel12B-WS was 11.8times higher than that by pET-20b-Cel12B. |
| |
| Keywords: | endoglucanase Thermotago maritime signal peptide expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
