菜籽饼粕中多肽的提取纯化及抗肿瘤活性

从菜籽饼粕中分离纯化多肽,并验证其抗肿瘤活性.采用超声-微波协同萃取法获得菜籽饼粕总蛋白,通过碱性蛋白酶水解提取菜籽饼粕总多肽,连用分子排阻色谱和反相高效液相色谱纯化获得菜籽饼粕多肽(RMP).通过对Hela细胞生长的抑制作用评价RMP的抗肿瘤活性.结果 显示:RMP由8个氨基酸组成,序列为Asp-Val-Phe-Va...

Extraction, purification and anti-tumor activity of a novel peptide from rapeseed meal

This article mainly studied the extraction, purification and anti-tumor activity of a novel peptide (RMP) isolated from the rapeseed meal. The total protein of rapeseed meal was obtained by ultrasonic-microwave synergistic extraction method and the total peptides were got by alkaline protease hydrolysis. RMP were separated by molecular exclusion chromatography from the total peptides and purified by reversed-phase high performance liquid chromatography (RP-HPLC). Structural analysis showed that the molecular mass of RMP was 886 Da, and it has a simple amino acid sequence: Asp-Val-Phe-Val-Pro-Pro-Val-Leu. To explore the antitumor activity of RMP, the effect of RMP on Hela cell apoptosis and the underlying preventative mechanisms were investigated. Results showed that RMP reduced the viability of Hela cells in a concentration-dependent manner. TUNEL analysis and flow cytometric examination showed that RMP decreased cell proliferation via a mitochondrial-dependent pathway, as promotion of the movement of cytochrome C from the mitochondria to the cytoplasm, and triggering of Caspase-9 and -3. Therefore, RMP may be a promising tumor preventive agent, can be used in health care products and medicines for treatment of tumors.