Metabolism of [1-14C]docosahexaenoate (22∶6n−3), [1-14C]eicosapentaenoate (20∶5n−3) and [1-14C]linolenate (18∶3n−3) in brain cells from juvenile turbotScophthalmus maximus |
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Authors: | Douglas R. Tocher Gabriel Mourente John R. Sargent |
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Affiliation: | (1) NERC Unit of Aquatic Biochemistry, School of Natural Sciences, University of Stirling, FK9 4LA Stirling, Scotland, UK;(2) Present address: Departmento de Biología, Facultad de Ciecias del Mar, Universidad de Cádiz, Rio San Pedro, E-11510 Puerto Real (Cádiz), Spain |
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Abstract: | The incorporation of [1-14C]18∶3n−3, (LNA) and [1-14C]-22∶6n−3 (DHA), and the metabolismvia the desaturase/elongase pathways of [1-14C]LNA, and [1-14C]20∶5n−3 (EPA) were studied in brain cells from newly-weaned (1-month-old) and 4-month-old turbot. The rank order of the extent of net incorporation of both LNA and DHA into glycerophospholipids was total diradyl glycerophosphocholines (CPL)> total diradyl glycerophosphoethanolamines (EPL)> phosphatidylserine (PS) and phosphatidylinositol (PI) and was independent of the polyunsaturated fatty acid added, the age of the fish and the time of incubation. However, the rate of incorporation of LNA into total lipid, CPL, EPL and PS was significantly greater than the rate of incorporation of DHA, and there was a significantly greater amount of DHA incorporated into EPL than LNA. There was no significant difference between the amounts of LNA and DHA incorporated into total lipid, CPL, PS and PI. Therefore, little preferential uptake and incorporation of DHA into brain cells was apparent. In 24-h incubations, on average 1.1% and 8.5% of radioactivity from [1-14C]LNA and [1-14C]EPA, respectively, were recovered in the DHA fraction. Therefore, LNA cannot contribute significantly to brain DHA levels in the turbot but EPA can. There were no significant differences between the amounts of radioactivity from either [1-14C]LNA or [1-14C]EPA recovered in the individual products/intermediates of the desaturase pathways in brain cells from 30-day-old and 120-day-old turbot. |
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