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Impact of culture source and linoleic acid (C18:2) on biohydrogen production from glucose under mesophilic conditions |
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| Authors: | Noori M. Cata Saady Subba Rao Chaganti Jerald A. Lalman Daniel Heath |
| Affiliation: | 1. Department of Civil and Environmental Engineering, University of Windsor, 205 Essex Hall, 401 Sunset Ave., Windsor, ON N9B 3P4, Canada;2. Great Lakes Institute for Environmental Research, University of Windsor, 401 Sunset Ave., Windsor, ON N9B 3P4, Canada;3. Department of Civil and Environmental Engineering, University of Windsor, 334 Essex Hall, 401 Sunset Ave., Windsor, ON N9B 3P4, Canada;4. Great Lakes Institute for Environmental Research and the Department of Biological Sciences, University of Windsor, 401 Sunset Ave., Windsor, ON N9B 3P4, Canada |
| Abstract: | Genomic and statistical methods were used to demonstrate the effects of linoleic acid (LA) on hydrogen (H2) production in mixed anaerobic cultures from two sources (designated as A and B). The microbial composition of the control cultures CA and CB were statistically different. Bacteroidaceae (26%) and Clostridiaceae (10%) dominated CA whereas Clostridiaceae (33%) and Bacteroidaceae (10%) dominated CB. Homoacetogens directed 42% of the electron equivalents to acetate production and decreased the H2 yield by 50% in CA compared to CB. The maximum H2 yields (3.11 ± 0.02 and 3.11 ± 0.07 mol H2 mol−1 glucose in LA-treated cultures ALA and BLA, respectively) were statistically the same. Cultures ALA and BLA followed the acetate-butyrate pathway while CA and CB followed propionate and homoacetogenic pathways. LA-treated and control cultures were statistically different based on the type and quantity of metabolites; the differences were also confirmed by principal component analysis (PCA). |
| Keywords: | Biohydrogen Linoleic acid (C18:2) Anaerobic Mixed culture Principal component analysis (PCA) T-RFLP |
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