Enhanced photo-hydrogen production of Rhodopseudomonas faecalis RLD-53 by EDTA addition |
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Authors: | Hong-Yu Ren Bing-Feng LiuJun Nan Guo-Jun XieLei Zhao Ming-Guang ChenNan-Qi Ren |
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Affiliation: | State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, P.O. Box 2614, 73 Huanghe Road, Harbin 150090, China |
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Abstract: | This study investigated the effect of EDTA concentration in medium on the growth, hydrogen production and nitrogenase activity of Rhodopseudomonas faecalis RLD-53 by batch cultures. Experimental results indicated that bacterial growth and hydrogen production were strongly inhibited with EDTA concentration increasing to 0.6–0.7 g/L. However, the lag time of hydrogen production and the trends of biomass at EDTA concentration of 0–0.5 g/L were similar. The maximum cumulative hydrogen volume of 3325 ml H2/L culture, hydrogen production rate of 27.6 ml H2/L/h and hydrogen yield of 2.97 mol H2/mol acetate were obtained when EDTA concentration was at 0.3 g/L, and the maximum OD660 attained 3.83. And the nitrogenase activity also reached a maximum value of 1331.9 μl-C2H4/h/mg dry weight in the medium containing Fe2+ and EDTA. These results showed that a proper concentration of EDTA can promote the availability of iron, thereby further enhancing the activity of nitrogenase and photo-hydrogen production. |
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Keywords: | Hydrogen production EDTA Iron Photo-fermentation Nitrogenase activity |
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