| 酶法有限水解米渣蛋白动力学研究 |
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| 引用本文： | 李积华,郑为完,张斌,苏冰霞. 酶法有限水解米渣蛋白动力学研究[J]. 食品科技, 2007, 32(4): 234-237 |
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| 作者姓名： | 李积华 郑为完 张斌 苏冰霞 |
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| 作者单位： | 南昌大学食品科学教育部重点实验室,南昌,330047 |
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| 基金项目： | 教育部长江学者和创新团队发展计划 |
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| 摘 要： | 采用pH-stat法对Alcalase 2.4L FG酶有限水解米渣蛋白动力学特性进行了探讨。探讨了温度、酶浓度(mL/120mL水)、底物浓度(g渣/120mL水)、pH值和水解时间对水解度的影响,并确定了比较理想的工艺参数;探讨了该酶催化水解米渣蛋白反应的动力学特性,并在理论上计算出临界底物浓度为22.25g渣/120mL水;建立有限水解动力学模型,验证实验结果表明,模型预测的最大相对误差小于10%。
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| 关 键 词： | 米渣 蛋白 酶解 动力学 |
| 文章编号： | 1005-9989(2007)04-0234-03 |
| 修稿时间： | 2006-09-06 |
Study on the kinetics of limited hydrolysis on rice residue with Alcalase 2.4L FG |
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| LI Ji-hua,ZHENG Wei-wan,ZHANG Bin,SU Bing-xia. Study on the kinetics of limited hydrolysis on rice residue with Alcalase 2.4L FG[J]. Food Science and Technology, 2007, 32(4): 234-237 |
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| Authors: | LI Ji-hua ZHENG Wei-wan ZHANG Bin SU Bing-xia |
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| Abstract: | This paper has investigated the kinetics of limited hydrolysis on rice residue with Alcalase 2.4L FG by pH-stat method.The factors including reaction temperature,enzyme concentration,substrate concentration,pH value and reaction time were discussed and figged out for the reaction.Discussed the kinetics characteristics of the reaction catalyzed by the enzyme.The critical substrate concentration was worked out theoretically as 22.25g residue per 120mL water.The hydrolysis kinetics model was established and proved accurate.The RSD is within 10% in test experiments. |
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| Keywords: | rice residue protein enzymatic hydrolysis kinetics |
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