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A solid chemical oxygen demand (COD) method for determining biomass in waste waters
Affiliation:1. Faculty of Environmental Management, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand;2. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand;3. Department of Civil, Construction and Environmental Engineering, Iowa State University, Ames, IA 50011-3232, USA;4. Energy Technology Research Center, Department of Civil Engineering, Faculty of Engineering, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand;1. The Catholic University of America, Washington, DC, USA;2. CH2M Hill, USA;3. DC Water, Washington, DC, USA;4. Hampton Roads Sanitation District, VA, USA;5. Dynamita, Nyons, France;6. ARAconsult, Innsbruck, Austria;1. Department of Environmental Engineering, Technical University of Denmark, Kgs. Lyngby DK-2800, Denmark;2. Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padua, viale dell''Università 16, 35020 Legnaro (PD), Italy;3. Department of Biology, University of Padua, Via U. Bassi 58/b, 35121 Padova, Italy;1. EPSRC Centre for Doctoral Training in Sustainable Chemical Technologies, University of Bath, Claverton Down, Bath BA2 7AY, UK;2. Department of Chemical Engineering, University of Bath, Claverton Down, Bath BA2 7AY, UK;3. Centre for Sustainable and Circular Technologies (CSCT), University of Bath, Claverton Down, Bath BA2 7AY, UK;4. Water Innovation & Research Centre (WIRC), University of Bath, Claverton Down, Bath BA2 7AY, UK;5. Department of Biology & Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, UK;1. Embrapa Suínos e Aves, BR 153 km 110, 89700-000 Concórdia, SC, Brazil;2. Universidade do Oeste de Santa Catarina – UNOESC, 89560-000 Videira, SC, Brazil;3. Universidade Estadual do Oeste do Paraná – UNIOESTE/CCET/PGEAGRI, 85819110, Cascavel, PR, Brazil;4. Universidade Federal de Santa Catarina – UFSC, Departamento de Engenharia Química, 88034001 Florianópolis, SC, Brazil
Abstract:A method of biomass determination similar to volatile suspended solids (VSS) that used chemical oxygen demand (COD) as a detection method instead of gravimetric methods is described. The “solid COD” method has a strong correlation with VSS for biomass at different stages of growth on different wastewaters including municipal sewage and bleached kraft mill effluent. Soluble COD from the wastewaters did not interfere with the assay. However, non-biomass suspended solids in chemi-thermo-mechanical pulping effluent were found to have a different COD/VSS ratio and thus interfered with solid COD results. The ratio between COD and VSS for biomass was approx. 1.2–1.6 mg COD/mg VSS.
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