Characterization of an ammonium transport protein from the peribacteroid membrane of soybean nodules |
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Authors: | BN Kaiser PM Finnegan SD Tyerman LF Whitehead FJ Bergersen DA Day MK Udvardi |
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Affiliation: | Division of Biochemistry and Molecular Biology, The Australian National University, Canberra ACT 0200, Australia. |
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Abstract: | Nitrogen-fixing bacteroids in legume root nodules are surrounded by the plant-derived peribacteroid membrane, which controls nutrient transfer between the symbionts. A nodule complementary DNA (GmSAT1) encoding an ammonium transporter has been isolated from soybean. GmSAT1 is preferentially transcribed in nodules and immunoblotting indicates that GmSAT1 is located on the peribacteroid membrane. [14C]methylammonium uptake and patch-clamp analysis of yeast expressing GmSAT1 demonstrated that it shares properties with a soybean peribacteroid membrane NH4+ channel described elsewhere. GmSAT1 is likely to be involved in the transfer of fixed nitrogen from the bacteroid to the host. |
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