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Inhibition of fish oil oxidation and the radical scavenging activity of New Zealand seaweed extracts
Authors:Sophie Kindleysides  Siew-Young Quek  Matthew R Miller
Affiliation:1. Food Science, School of Chemical Sciences, The University of Auckland, 23 Symonds St., Auckland, New Zealand;2. Natural Extracts Team, The New Zealand Institute for Plant & Food Research Limited, 300 Wakefield Quay, Nelson, New Zealand
Abstract:This study describes the potential use of New Zealand seaweed extracts as antioxidants in fish oils. The extracts from two brown seaweeds (Ecklonia radiata, Macrocystis pyrifera) and two red species (Champia sp. and Porphyra sp.), were added to hoki (Macruronus novaezelandiae) oil and assessed by production of oxidation products in an elevated temperature (60 °C) storage trial. Antioxidant assays ORAC and DPPH were conducted to elucidate the radical scavenging activity of the seaweed extracts. The results showed that all the lipid-soluble seaweed extracts had some antioxidative capacity, with brown algae generally outperforming red algae species. Extracts from E. radiata performed best with significantly lower primary, secondary and total oxidation products, and higher DPPH radical scavenging ability than the commercial antioxidant, BHT. This study demonstrated the potential of brown algae seaweed extracts, in particular E. radiata, for use as antioxidants in fish oil products.
Keywords:AAPH, 2,2&prime  -azobis (2-amidinopropane) dihydrochloride   AOCS, American Oil Chemists&rsquo   Society   ANOVA, 1-way analysis of variance   BHA, butylated hydroxyanisole   BHT, butylated hydroxytoluene   DHA, docosahexaenoic acid   DPA, docosapentaenoic acid   DPPH, 1,1-diphenyl-2-picrylhydrazyl   EPA, eicosapentaenoic acid   FA, fatty acid(s)   FAMEs, fatty acid methyl ester(s)   GC, gas chromatograph   HSD, honestly significant difference   LCPUFA, long chain (C     20) polyunsaturated fatty acid(s)   meq, milliequivalent   MUFA, monounsaturated fatty acid(s)p   n&minus  3, Omega 3   n&minus  6, Omega 6   nm, nanometre   OD, optical density   ORAC, Oxygen Radical Antioxidant Capacity   pAV, para-anisidine value   PG, propyl gallate   PV, peroxide value   SFA, saturated fatty acid(s)   SPSS, Statistical Package for the Social Sciences   TBHQ, tert-butylhydroxyquinone
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