Dioscin: A synergistic tyrosinase inhibitor from the roots of Smilax china |
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Authors: | Chun Liang Ju-Hwan Lim Seung-Hyung Kim Dong-Seon Kim |
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Affiliation: | 1. TKM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea;2. Natural Bio R&D Center, Sun Bio Tech Co., Ltd., Daejeon 305-509, Republic of Korea;3. Institute of Traditional Medicine & Bioscience, Daejeon University, Daejeon 300-716, Republic of Korea;4. Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea |
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Abstract: | In our study, we investigated the inhibition of mushroom tyrosinase in Smilax china. A methanol (MeOH) extract of S. china was partitioned into hexane, ethyl acetate (EtOAc) and water. Of the three fractions, EtOAc extract showed the strongest inhibition of tyrosinase activity with l-tyrosine or l-DOPA as a substrate. Two compounds were isolated from a final active fraction by activity-guided column chromatography. These compounds were identified as dioscin and oxyresveratrol by comparing their mass, 1H- and 13C-NMR spectral data with those reported in the literature. Dioscin showed little inhibition activity of tyrosinase, whereas oxyresveratrol, a known tyrosinase inhibitor, showed a strong tyrosinase inhibitory activity. We discovered that a mixture of oxyresveratrol and dioscin (IC50 = 5.1 and 5.7 μg/ml) highly increased the inhibition of tyrosinase activity with l-tyrosine or l-DOPA as the substrate as compared to either oxyresveratrol (IC50 = 7.8 and 10.9 μg/ml) or dioscin (IC50 > 100 and 100 μg/ml) alone. |
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Keywords: | Smilax china Tyrosinase inhibitor Oxyresveratrol Dioscin Synergistic |
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