Body weight management effect of burdock (Arctium lappa L.) root is associated with the activation of AMP-activated protein kinase in human HepG2 cells |
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| Authors: | Daih-Huang Kuo Ming-Chi Hung Chao-Ming Hung Li-Min Liu Fu-An Chen Po-Chuen Shieh Chi-Tang Ho Tzong-Der Way |
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| Affiliation: | 1. Department of Pharmacy, Graduate Institute of Pharmaceutical Technology, Tajen University, Pingtung, Taiwan, ROC;2. Department of General Surgery, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan, ROC;3. Graduate Institute of Pharmaceutical Chemistry, College of Pharmacy, China Medical University, Taichung, Taiwan, ROC;4. Department of Food Science, Rutgers University, New Brunswick, NJ, USA;5. Department of Biological Science and Technology, College of Life Sciences, China Medical University, Taichung, Taiwan, ROC;6. Institute of Biochemistry, College of Life Science, National Chung Hsing University, Taichung, Taiwan, ROC |
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| Abstract: | Burdock (Arcticum lappa L.) root is used in folk medicine and also as a vegetable in Asian countries. In the present study, burdock root treatment significantly reduced body weight in rats. To evaluate the bioactive compounds, we successively extracted the burdock root with ethanol (AL-1), and fractionated it with n-hexane (AL-2), ethyl acetate (AL-3), n-butanol (AL-4), and water (AL-5). Among these fractions, AL-2 contained components with the most effective hypolipidemic potential in human hepatoma HepG2 cells. AL-2 decreased the expression of fatty acid synthase (FASN) and inhibited the activity of acetyl-coenzyme A carboxylase (ACC) by stimulating AMP-activated protein kinase (AMPK) through the LKB1 pathway. Three active compounds were identified from the AL-2, namely α-linolenic acid, methyl α-linolenate, and methyl oleate. These results suggest that burdock root is expected to be useful for body weight management. |
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| Keywords: | Body weight management Burdock root FASN ACC AMPK |
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