Bioactivity evaluations of ingredients extracted from the flowers of Citrus aurantium L. var. amara Engl |
| |
Authors: | Hai-Yan Zhao Li Yang Jian Wei Ming Huang Jian-Guo Jiang |
| |
Affiliation: | 1. College of Food and Bioengineering, South China University of Technology, Guangzhou 510640, China;2. Guangzhou Baidi Biotechnology Co., Ltd., Guangzhou 510640, China;3. Xinjiang Lvxiang sugar Co., Ltd., Xinjiang 834601, China |
| |
Abstract: | Citrus aurantium L. var. amara Engl, a member of genus Citrus (Rutaceae), widespread in China, is used as folk medicine for the treatment of helping digest, phlegm, enteritidis, stomachic and other deceases. In the present research, silica gel column, Sephadex LH-20, mass spectrometer (MS) and nuclear magnetic resonance (NMR) were used to separate and identify the chemical compounds from the flowers of C. aurantium var. amara, and several bioactivity assays were used to evaluate their antioxidant, anti-inflammatory, anti-virus and antitumour activities. Two major compounds, 5-hydroxy-6,7,3′,4′-tetramethoxyflavone (HTF) and limonexic acid (LA), were isolated and identified from C. aurantium var. amara for the first time. The results of the bioactivity assays showed that HTF and LA displayed significant antioxidant activities and showed significant inhibition effects on the B16 cell lines at a concentration range from 6.25 to 50 μg/ml, and on the SMCC-7721 cell lines from 12.5 to 200 μg/ml. The antitumour effect, anti-inflammatory activity and the inhibiting expression of HBsAg and HBeAg of 2.2.15 cells displayed the tendency in a concentration-dependent manner. These two compounds from C. aurantium var. amara could potentially be used as a promising natural agent in the pharmaceutical industries. |
| |
Keywords: | Extraction Anti-oxidant activity Cytotoxic activity Anti-inflammatory activity Anti-virus 5-Hydroxy-6 7 3&prime 4&prime -tetramethoxyflavone Limonexic acid |
本文献已被 ScienceDirect 等数据库收录! |
|