Effect of flanking sequences on the right- to left-handed transition of a (dA-dT)n tract in supercoiled DNA |
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Authors: | K Nejedly DM Lilley E Palecek |
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Affiliation: | Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic. |
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Abstract: | Alternating (dA-dT)n sequences in supercoiled DNA may undergo a transition to a left-handed conformation in the presence of Ni2+ ions and high NaCl concentration (Nejedly, K., Klysik, J. and Palecek, E., FEBS Lett. 243, 313-317 (1989)). In this work we have found that ionic conditions necessary for the B-to-Z transition are strongly dependent on the sequences flanking the (dA-dT)n tract. In particular, the presence of 5'-homopyrimidine (C3) and 3'-homopurine (G4) blocks adjacent to the tract were found to facilitate the transition to the left-handed form. Within a constant sequence context it was found that the ionic strength required to promote the transition was inversely proportional to the length of the (dA-dT)n sequence. |
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