Detection of milk powder and caseinates in Halloumi cheese |
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Authors: | L Pellegrino S Cattaneo G Psathas |
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Affiliation: | * Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Università degli Studi di Milano, I-20133 Milano, Italy † The Cyprus Milk Industry Organisation, Nicosia, CY-1900 Cyprus |
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Abstract: | Halloumi cheese is traditionally manufactured from fresh milk. Nevertheless, dried dairy ingredients are sometimes illegally added to increase cheese yield. Lysinoalanine and furosine are newly formed molecules generated by heating and drying milk protein components. The levels of these molecular markers in the finished Halloumi have been investigated to verify their suitability to reveal the addition of skim milk powder and calcium caseinate to cheese milk. Because of the severe heating conditions applied in curd cooking, genuine Halloumi cheeses (n = 35), representative of the Cyprus production, were characterized by levels of lysinoalanine (mean value = 8.1 mg/100 g of protein) and furosine (mean value = 123 mg/100 g of protein) unusual for natural cheeses. Despite the variability of the values, a good correlation between the 2 parameters (R = 0.975) has been found in all cheeses, considering both the fresh and mature cheeses as well as those obtained from curd submitted to a prolonged cooking following a traditional practice adopted by a very small number of manufacturers. Experimental cheeses made by adding as low as 5% of skim milk powder, or calcium caseinate, or both, to cheese milk fell outside the prediction limits at ±2 standard deviation of the above-reported correlation regardless of curd cooking conditions or ripening length. This correlation may be adopted as a reliable index of Halloumi cheese genuineness. |
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Keywords: | Halloumi cheese cheese genuineness furosine lysinoalanine |
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