Surface topography effect on fibroblasts population on epiclon-based polyimide films |
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| Authors: | Iuliana Stoica Andreea Irina Barzic Maria Butnaru Florica Doroftei Camelia Hulubei |
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| Affiliation: | 1. Department of Polymer Materials Physics, ‘Petru Poni’ Institute of Macromolecular Chemistry, Grigore Ghica Voda Alley 41A, 700487 Iasi, Romania;2. Department of Bioresources and Polymer Science, Faculty of Applied Chemistry and Materials Science, University POLITEHNICA of Bucharest, str. Gheorghe Polizu, nr 1-7, Sector 1, 011061 Bucharest, Romaniastoica_iuliana@icmpp.ro;4. Department of Physical Chemistry of Polymers, ‘Petru Poni’ Institute of Macromolecular Chemistry, Grigore Ghica Voda Alley 41A, 700487 Iasi, Romania;5. Department of Biomedical Sciences, Faculty of Medical Bioengineering, ‘Grigore T. Popa’ University of Medicine and Pharmacy, University Street 16, 700115 Iasi, Romania;6. Department of Polycondensation and Thermostable Polymers, ‘Petru Poni’ Institute of Macromolecular Chemistry, Grigore Ghica Voda Alley 41A, 700487 Iasi, Romania |
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| Abstract: | A polyimide based on alicyclic units, such as epiclon (5-(2,5-dioxotetrahydrofurfuryl)-3-methyl-3-cyclohexene-1,2-dicarboxylic acid anhydride), was investigated from the point of view of its surface features to check their suitability for cell culture applications. Atomic force microscopy data revealed fractal and isotropic surfaces with nanoscale roughness and peaks placed at much smaller distances comparing to the cell size scale, favoring a good compatibility of the synthesized material with the biological medium, particularly after plasma treatment. Surface tension properties were determined in order to evaluate the interactions at the bio-interface affecting the adherence behavior of cell-binding proteins to the sample surface. In vitro experiments regarding the cytocompatibility and population tendency reveal that polyimide allows cells to adhere and to proliferate onto the surface. These tests indicate that the studied epiclon-based polyimide is not cytotoxic and can be recommended as good candidate for cell culture substrate in tissue engineering, especially after plasma treatment. |
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| Keywords: | alicyclic polyimide morphology texture parameters fibroblasts adhesion |
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