| 大孔树脂纯化山杏核壳总黄酮的工艺优化 |
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| 引用本文： | 杨 喆,万 山,张乔会,宁亚萍,董施彬,王建中.大孔树脂纯化山杏核壳总黄酮的工艺优化[J].食品科学,2015,36(10):38-42. |
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| 作者姓名： | 杨 喆 万 山 张乔会 宁亚萍 董施彬 王建中 |
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| 作者单位： | 北京林业大学 林业食品加工与安全北京市重点实验室，北京 100083 |
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| 基金项目： | 林业公益性行业科研专项（201004081） |
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| 摘 要： | 以山杏核壳为原料，采用大孔树脂纯化山杏核壳提取液中的总黄酮，利用单因素试验和正交试验优化D101大孔树脂纯化山杏核壳总黄酮的工艺条件。结果表明：在上样溶液质量浓度0.3 mg/mL、pH 2、流速2 mL/min的条件下进行纯化实验，大孔树脂对山杏核壳总黄酮的平均吸附率为75.20%。山杏核壳总黄酮的质量分数可达51.63%，表明D101大孔树脂对山杏核壳总黄酮有较好的纯化效果，且工艺重复性和稳定性良好。
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| 关 键 词： | 山杏核壳 总黄酮 大孔树脂 纯化 |
Purification of Total Flavonoids from Shells of Wild Apricot with Macroreticular Resin |
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| YANG Zhe,WAN Shan,ZHANG Qiaohui,NING Yaping,DONG Shibin,WANG Jianzhong.Purification of Total Flavonoids from Shells of Wild Apricot with Macroreticular Resin[J].Food Science,2015,36(10):38-42. |
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| Authors: | YANG Zhe WAN Shan ZHANG Qiaohui NING Yaping DONG Shibin WANG Jianzhong |
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| Affiliation: | Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China |
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| Abstract: | The purification of total flavonoids extracted from the shells of wild apricot using D101 macroreticular resin
was optimized using combination of single factor and orthogonal array experiments. The results showed that the optimum
purification conditions were determined as follows: sample concentration, 0.3 mg/mL; pH 2; flow rate for elution,
2 mL/min. With these parameters, the purity of total flavonoids was 51.63%. Therefore, D101 macroreticular resin was
effective in purifying total flavonoids from the shells of wild apricot with good stability and repetition. |
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| Keywords: | shells of wild apricot total flavonoids macroreticular resin purification |
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