沙棘籽渣酶解产物的体内外抑菌作用

以沙棘籽渣为原料，采用水提法提取沙棘籽渣蛋白，再用ProteAX复合蛋白酶和胃蛋白酶两种酶酶解，利用膜过滤对其分离纯化，得到具有抑菌活性的沙棘籽渣蛋白酶解物。体外实验结果表明：ProteAX复合蛋白酶水解多肽对金黄色葡萄球菌、沙门氏菌、绿脓杆菌均有抑制作用，胃蛋白酶水解多肽对大肠杆菌、金黄色葡萄球菌、沙门氏菌有抑制作用。动物实验表明：胃蛋白酶水解多肽灌胃的小鼠粪便中，沙门氏菌的菌落总数与阳性对照组相比显著减少（P＜0.05）；而灌胃ProteAX复合蛋白酶水解多肽的小鼠粪便中，金黄色葡萄球菌的菌落总数比阳性对照组显著降低（P＜0.05）。

Antibacterial Activity in vivo and in vitro of Enzymatic Hydrolysates of Sea Buckthorn Seed Residue

In this work, we obtained antibacterial peptides by enzymatic hydrolysis of water-soluble proteins from the
residue left after supercritical carbon dioxide extraction of seed oil from sea buckthorn seeds with ProteAX protease and
pepsin respectively and purification of the resultant hydrolysates by membrane filtration. in vitro test results indicated that
the ProteAX hydrolysate possessed bacteriostasis on Staphylococcus aureus, Salmonella, and Pseudomonas aeruginosa,
while the pepsin hydrolysate possessed bacteriostasis on Escherichia coli, Staphylococcus aureus and Salmonella. Animal
test results showed that the count of Salmonella in the feces of mice administered by gavage with the pepsin hydrolysate was
significantly reduced (P < 0.05) when compared with the positive group, whereas the count of Staphylococcus aureus in the
feces of mice orally administered with the ProteAX hydrolysate was significantly lower (P < 0.05) when compared with the
positive group.