药桑多糖对四氯化碳所致大鼠肝损伤的保护作用

目的：研究药桑多糖对四氯化碳（CCl4）所致大鼠肝损伤的保护作用。方法：取健康Sprague-Dawley（SD）大鼠40 只，根据体质量随机分为5 组：对照组、CCl4损伤组、药桑多糖低（50 mg/（kg•d））、中（100 mg/（kg•d））、高（200 mg/（kg•d））剂量组，每组8 只，连续灌胃7 d后，除对照组外，其余各组大鼠腹腔注射CCl4橄榄油溶液制造肝损伤模型。24 h后，取血清测定谷丙转氨酶（alanine aminotransferase，ALT）、谷草转氨酶（aspartate aminotransferase，AST）活力以及胆红素含量，取各器官计算脏器指数并测定肝脏超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）、谷胱甘肽过氧化物酶（glutathionperoxidase，GSH-Px）活力，以及丙二醛（malondialdehyde，MDA）、干扰素-γ（interferon-γ，IFN-γ）、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、白细胞介素-10（interleukin-10，IL-10）含量，并对肝脏进行组织切片观察。结果：100、200 mg/（kg•d）剂量的药桑多糖能显著增加大鼠体质量（P＜0.05），降低肝脏指数（P＜0.05）、肾脏指数（P＜0.05）和脾脏指数（P＜0.01），能显著抑制CCl4所致肝损伤大鼠血清ALT、AST活力和胆红素含量的升高（P＜0.05）；肝脏MDA、IFN-γ、TNF-α含量显著降低（P＜0.05），SOD、CAT、GSH-Px活力以及IL-10含量显著升高（P＜0.05）。50 mg/（kg•d）剂量的药桑多糖能显著降低肝损伤大鼠的肝脏指数、肾脏指数和脾脏指数（P＜0.05），显著增加肝脏GSH-Px活力和IL-10含量（P＜0.05），降低肝脏TNF-α含量（P＜0.05）。结论：药桑多糖对CCl4诱导的大鼠肝损伤具有明显的保护作用，其肝脏保护作用与提高肝脏抗氧化能力及抑制肝脏炎症有关。

Protective Effect of Polysaccharides from Morus nigra Linn.Fruits on CCl4-Induced Liver Damage in Rats

Objective: To study the hepatoprotective effect of polysaccharides from Morus nigra Linn. fruits (MNP) on
CCl4-induced liver damage in rats. Methods: Totally 40 healthy SD rats were randomized into 5 groups with 8 animals in
each group: normal group, CCl4-injury group, and three different treatment groups with different doses of MNP (50, 100,
200 mg/(kg·d)). After 7 days of intragastric administration, all rats except for the normal group were subjected
to intraperitoneal injection of CCl4. After 24 hours, rat serum was collected for analyzing the activity of alanine
aminotransferase (ALT) and aspartate aminotransferase (AST), and the content of bilirubin. Liver tissues were harvested
for measuring the activities of superoxide dismutase (SOD), catalase (CAT) and glutathion peroxidase (GSH-Px), and the
contents of malondialdehyde (MDA), interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10).
Results: Compared with the CCl4-injury group, the treatment groups with 100 and 200 mg/(kg·d) of MNP revealed a
significant decrease in body weight (P < 0.05), liver index (P < 0.05), kidney index (P < 0.05) and spleen index (P < 0.01);
serum ALT and AST activities (P < 0.05) and bilirubin content (P < 0.05); and the contents of MDA, IFN-γ and TNF-α
(P < 0.05) in liver, while the activities of liver SOD, CAT, and GSH-Px and IL-10 contentwere significantly increased (P < 0.05).
The administration with 50 mg/(kg·d) of MNP could result in a significant decrease in liver, kidney and spleen indexes (P < 0.05),
GSH-Px activity (P < 0.05), IL-10 content (P < 0.05) in liver, and TNF-α content (P < 0.05) in liver. Conclusion: Polysaccharides
from Morus nigra Linn. fruits can attenuate CCl4-injduced liver injury in rats, and the underlying mechanism may be due to its
antioxidant and anti-inflammatory effects.