气相色谱-串联质谱法快速测定水果中咪鲜胺及其代谢物2,4,6-三氯苯酚的残留量

目的：建立基质分散固相萃取-气相色谱-串联质谱法快速测定水果中咪鲜胺及其代谢物2,4,6-三氯苯酚残留量的方法。方法：采用以乙腈作提取剂、以N-丙基乙二胺和十八烷基硅烷键合硅胶（C18）作为混合固相基质分散萃取净化剂的前处理方法，并利用气相色谱-串联质谱在多反应离子监测模式条件下进行检测，用基质匹配标准品的外标法定量。结果：咪鲜胺及其代谢物2,4,6-三氯苯酚在0.004～0.400 mg/kg范围内线性关系均良好，相关系数分别为0.999 4和0.999 2；在3 个添加水平（0.01、0.02、0.10 mg/kg）范围内的平均回收率分别为80.1%～101.5%和79.0%～97.4%，相对标准偏差分别为2.8%～6.8%和2.0%～6.0%，方法的定量限分别为0.004 mg/kg和0.005 mg/kg。结论：该方法前处理简单快速、灵敏度高、测定线性范围宽，可用于水果中咪鲜胺及其代谢物2,4,6-三氯苯酚残留的快速测定。

Rapid Determination of Residues of Prochloraz and Its Metabolite 2,4,6-Trichlorophenol in Fruits Using Gas Chromatography with Tandem Mass Spectrometry

Purpose: To develop a rapid method for the determination of residues of prochloraz and its metabolite,
2,4,6-trichlorophenol, in fruits using matrix dispersive solid-phase extraction and gas chromatography with tandem mass
spectrometry (GC-MS-MS). Methods: Samples were extracted with acetonitrile, cleaned by matrix dispersive solid-phase
extraction using PSA and C18, detected by GC-MS-MS under multiple reaction monitoring mode, and quantified by an
external standard method using matrix-match standard solution. Results: In the linear range of 0.004-0.400 mg/kg, the
correlation coefficients for prochloraz and 2,4,6-trichlorophenol were 0.999 4 and 0.999 2, respectively. Their average
recoveries at spiked levels of 0.01, 0.02 and 0.10 mg/kg were 80.1%–101.5% and 79.0%-97.4% with relative standard
deviations of 2.8%–6.8% and 2.0%–6.0%, respectively, and their limits of quantification (LOQ) (RSN = 10) were 0.004 and
0.005 mg/kg, respectively. Conclusions: The proposed method is simple and sensitive with a wide range of measurement,
and can be suitable for the analysis of prochloraz and 2,4,6-trichlorophenol residues in fruits.