连翘花提取物活性成分及其稳定性

采用高效液相色谱方法对河南连翘花提取物中芦丁、金丝桃苷、连翘酯苷A、连翘苷、齐墩果酸、熊果酸含量进行分析，并对连翘花提取物稳定性进行研究。结果表明：连翘花提取物中含有较高含量的芦丁、金丝桃苷、连翘苷、齐墩果酸和熊果酸；在中性和弱酸性环境中连翘花提取物具有较强的稳定性；在25～75 ℃范围内，温度对连翘花提取物影响较小；室光和避光对连翘花提取物的影响小于自然光；金属离子K＋、Na＋、Ca2＋对连翘花提取物的吸光度影响较小，Mg2＋、Fe3＋有增强连翘花提取物吸光度作用；氧化剂、还原剂、蔗糖、食盐和淀粉对连翘花提取物稳定性影响较小。本实验为综合利用连翘花提取物资源提供有用信息。

Active Compounds and Stability of Forsythia suspensa Flower Extract

This study aimed to determine the contents of rutin, quercetin, forsythiaside A, phillyrin, oleanolic acid, and
ursolic acid in Forsythia suspensa flower extract (FFE) from Henan province by high performance liquid chromatography
(HPLC) and to evaluate the stability of FFE. The results showed that the contents of rutin, quercetin, phillyrin, oleanolic
acid, and ursolic acid in FFE were all higher. FFE was obviously stable between weakly acidic and neutral pH values, and
displayed a higher thermostability at temperatures ranging from 25 to 75 ℃. Natural light and dark treatments had less
effects on the extract than sunlight treatment. K+, Na+, and Ca2+ exerted less effect on the absorbance value whereas Mg2+ and
Fe3+ enhanced the absorbance value of FFE. Oxidants, reducing agents, sugar, NaCl, and starch had only slight effects on the
stability of FFE. This study can provide important information for comprehensive utilization of FFE.