壶瓶碎米荠中含硒蛋白结构特性及其缓解运动性疲劳的作用

目的：以纯化的壶瓶碎米荠含硒蛋白（selenium-containing protein from Cardamine hupingshanensis，SPCH）为研究对象，对其结构特性和缓解运动性疲劳作用进行评价。方法：采用聚丙烯酰胺凝胶电泳（polyacrylamide gelelectrophoresis，PAGE）和十二烷基硫酸钠-PAGE（sodium dodecyl sulfate-PAGE，SDS-PAGE）、氨基酸组成分析、基质辅助激光解吸电离飞行时间质谱（matrix-assisted laser desorption/ionizationtime of flight mass spectrometry，MALDI-TOF-MS）分析，对SPCH的纯度、亚基组成、氨基酸组成及含量进行评价并初步预测SPCH的匹配蛋白。采用小鼠负重游泳实验，通过检测游泳时间、血乳酸（blood lactic acid，BLA）、肝糖原和血尿素氮（blood urea nitrogen，BUN）水平，评价SPCH对小鼠运动性疲劳的影响。结果：SPCH可能由3 个分子质量分别为37、39、40 kD的亚基组成，与之相匹配的蛋白可能是DING protein、Predicted protein和Chalcone synthase。SPCH能显著延长小鼠负重游泳时间（P＜0.01），同时增强清除乳酸的能力（P＜0.01），增加肝糖原含量（P＜0.01），还具有降低BUN水平的能力（P＜0.05）。结论：SPCH对小鼠具有较好的缓解运动性疲劳作用，可考虑将其开发成为缓解运动性疲劳的营养补充剂。

Structure Characteristics and Anti-motor Fatigue Activity of Selenium-Containing Protein from Cardamine hupingshanensis

This study evaluated the structure characteristics and anti-motor fatigue activity of selenium-containing protein
purified from Cardamine hupingshanensis (SPCH). The purity, subunit composition, amino acid composition and matching
protein of SPCH were analyzed by using PAGE, SDS-PAGE, amino acid analyzer and matrix-assisted laser desorption/
ionization time of flight mass spectrometry (MALDI-TOF-MS). The anti-motor fatigue activity of SPCH was investigated by
measuring swimming time, blood lactic acid, hepatic glycogen and serum urea nitrogen in mice in weight-loaded swimming
test. Results showed that SPCH had three subunits with molecular weights of 37, 39 and 40 kD respectively. The matching
protein might be DING protein, predicted protein or chalcone synthase. The results also showed that SPCH significantly
prolonged the swimming time of mice (P < 0.01), decreased blood lactic acid content (P < 0.01), increased hepatic glycogen
content (P < 0.01), and reduced serum urea nitrogen content (P < 0.05). In conclusion, SPCH has a good anti-motor fatigue
activity as a dietary supplement.