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几种氧载体对纳他霉素发酵的影响
引用本文:王 爽,周 巍,刘红冉,李永波,马超峰,张 岩.几种氧载体对纳他霉素发酵的影响[J].食品科学,2015,36(9):102-107.
作者姓名:王 爽  周 巍  刘红冉  李永波  马超峰  张 岩
作者单位:1.河北省食品检验研究院,河北省食品安全重点实验室,河北 石家庄 050071; 2.河北农业大学食品科技学院,河北 保定 071000
摘    要:为有效改善纳他霉素发酵液中的氧传递速率,研究了几种氧载体对纳他霉素发酵的影响。结果表明,氧载体不能作为碳源被细胞利用,并能提高发酵体系溶氧量和传氧速率,有效促进单位细胞的纳他霉素产率的提高。在摇瓶发酵初期添加6%的大豆油、6%的橄榄油和9%的二甲基硅油,纳他霉素产量分别提高了56.8%、59.5%和72.5%。发酵初期添加6%的表面活性剂吐温-80可促进菌体生长,同时有效提高纳他霉素产量82.6%。

关 键 词:褐黄孢链霉菌  纳他霉素  氧载体  表面活性剂  

Effects of Oxygen Vectors on Microbial Production of Natamycin
WANG Shuang,ZHOU Wei,LIU Hongran,LI Yongbo,MA Chaofeng,ZHANG Yan.Effects of Oxygen Vectors on Microbial Production of Natamycin[J].Food Science,2015,36(9):102-107.
Authors:WANG Shuang  ZHOU Wei  LIU Hongran  LI Yongbo  MA Chaofeng  ZHANG Yan
Affiliation:1. Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050071, China; 2. College of Food Science and Technology, Agricultural University of Hebei, Baoding 071000, China
Abstract:In order to effectively improve the oxygen transfer rate in the microbial production of natamycin, the effect of
several oxygen vectors on Streptomyces gilvosporeus biomass and natamycin production was studied. The results showed
that oxygen vectors and surfactants could not be used as carbon source although they were useful to improve dissolved
oxygen and transfer rate, as well as the production of natamysin. The yield of natamycin could be improved by 56.8%,
59.5% and 72.5% after adding 6% soybean oil, 6% olive oil and 9% polydimethyls iloxane at the beginning of fermentation,
respectively. With the addition of 6% Tween-80, the yield of natamycin was improved by 82.6% and growth-promoting
effect on the strain was observed.
Keywords:Streptomyces gilvosporeus  natamycin  oxygen vector  surfactant  
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