反相高效液相色谱法测定东北地区6 种红树莓果实中鞣花酸含量

建立反相高效液相色谱法测定东北地区6 种红树莓果实中鞣花酸含量，同时与蓝莓进行比较分析。色谱条件：色谱柱SinoChrom DS-BP C18（150 mm×4.6 mm，5 μm）；流动相为CH3OH-0.3%磷酸（40∶60，V/V）；流速1 mL/min，柱温30 ℃，检测波长254 nm。结果表明：鞣花酸得到很好地分离，鞣花酸含量在0.1～1 μg范围内与峰面积呈良好的线性关系，R2为0.999 3。精密度实验相对标准偏差为0.06%，重复性实验相对标准偏差为0.09%，稳定性实验相对标准偏差为0.39%，平均加样回收率为93.00%～98.62%，相对标准偏差为0.21%～0.42%，该方法简单、准确、重复性好。测得不同品种红树莓果实中总鞣花酸含量为39.10～155.98 mg/100 g；游离鞣花酸含量为1.24～8.26 mg/100 g，红树莓中含有丰富的鞣花酸，具有潜在的开发利用价值。

Determination of Ellagic Acid from Six Red Raspberry Cultivars Grown in Northeast China Using RP-HPLC

A reversed phase high performance liquid chromatography (RP-HPLC) method was established to determine
the contents of ellagic acid in six red raspberry cultivars grown in northeast China. Meanwhile, the results were compared
with those of the blueberry fruit. A SinoChrom DS-BP C18 column (150 mm × 4.6 mm, 5 μm) was used to separate ellagic
acid; the mobile phase was a mixture of 0.3% phosphate and methanol solution (60:40, V/V) at a flow rate of 1.0 mL/min;
the column temperature was set at 30 ℃ and the UV detection wavelength was 254 nm. Our results indicated that ellagic
acid could be separated well, and a good linear relationship appeared between the content of ellagic acid in the range of 0.1–1 μg
and peak area with correlation coefficient of 0.999 3. The relative standard deviation (RSD) was 0.06% for precision, 0.09% for
reproducibility, and 0.39% for stability. The average recovery rates of ellagic acid from spiked samples were between 93.00% and
98.62% with RSD of 0.21%–0.42%. The proposed method is convenient, accurate, and repeatable. The contents of total ellagic
acid in different red raspberry cultivars varied from 39.10 to 155.98 mg/100 g, and the content of free ellagic acid was in the range
of 1.24 to 8.26 mg/100 g. Red raspberry is rich in ellagic acid, and it could be fully developed and utilized.