| 响应面优化双水相萃取分离酵母源MT工艺 |
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| 引用本文： | 王 月,张东杰,王 颖,梁小月,徐炳政.响应面优化双水相萃取分离酵母源MT工艺[J].食品科学,2015,36(10):54-58. |
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| 作者姓名： | 王 月 张东杰 王 颖 梁小月 徐炳政 |
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| 作者单位： | 1.黑龙江八一农垦大学食品学院，黑龙江 大庆 163319；2.国家杂粮技术工程研究中心，黑龙江 大庆 163319 |
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| 基金项目： | 黑龙江省教育厅新世纪人才项目（2014-2016）；黑龙江省博士后资助项目（LBH-Z13169） |
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| 摘 要： | 采用聚乙二醇（polyethylene glycol，PEG）-(NH4)2SO4双水相体系，分离铜诱导的诱变酿酒酵母菌所表达的金属硫蛋白。以PEG质量分数、(NH4)2SO4质量分数及pH值为考察因素，萃取率为响应值，结合Design-Expert 8.0.6软件做响应面优化分析，优化到最佳萃取条件：PEG相对分子质量2 000、PEG和(NH4)2SO4质量分数分别为22.5%和17.46%、pH 5.98。获取的金属硫蛋白主要分布在上相，萃取率可达81.69%。此方法操作简单、高效、成本低，是酵母源金属硫蛋白分离提纯的新方法。
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| 关 键 词： | 金属硫蛋白 双水相萃取 响应面分析 分配系数 萃取率 |
Optimization of Extraction Process of Yeast Metallothionein by Aqueous Two-Phase System |
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| WANG Yue,ZHANG Dongjie,WANG Ying,LIANG Xiaoyue,XU Bingzheng.Optimization of Extraction Process of Yeast Metallothionein by Aqueous Two-Phase System[J].Food Science,2015,36(10):54-58. |
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| Authors: | WANG Yue ZHANG Dongjie WANG Ying LIANG Xiaoyue XU Bingzheng |
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| Affiliation: | 1. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China;
2. National Coarse Cereals Engineering Research Center, Daqing 163319, China |
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| Abstract: | In this study, an aqueous two-phase system consisting of polyethylene glycol (PEG) and ammonium sulfate was
used to extract copper-induced metallothionein (MT) from a mutant strain of Saccharomyces cerevisiae. Response surface
methodology was employed to optimize three factors namely PEG concentration, (NH4)2SO4 concentration and pH that
influence extraction efficiency using the software Design-Expert 8.0.6. The optimal aqueous two-phase system for MT
extraction was determined to contain 22.5% PEG 2 000, 17.46% (NH4)2SO4 at pH 5.98. Under these optimal conditions, the
metallothionein was mainly distributed in the upper phase with an extraction rate of 81.69%. Therefore, this method can
provide a new, simple, convenient, economical and highly efficient strategy for the extraction of metallothionein from yeast. |
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| Keywords: | metallothionein aqueous two-phase system extraction response surface methodology distribution coefficient extraction rate |
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