| 荧光素酯的合成及对红豆杉细胞的染色作用 |
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| 引用本文： | 闫喜龙,王文锋,张素峰,李阳,白国义,陈立功. 荧光素酯的合成及对红豆杉细胞的染色作用[J]. 精细化工, 2005, 22(6): 440-443 |
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| 作者姓名： | 闫喜龙 王文锋 张素峰 李阳 白国义 陈立功 |
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| 作者单位： | 天津大学,化工学院,天津,300072;新乡市获嘉县中医院,河南,新乡,453801 |
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| 摘 要： | 以荧光素为原料,分别和丙烯酸、2-丁烯酸及-2-甲基-2-戊烯酸反应,合成了荧光素的二丙烯酸酯、二-2-丁烯酸酯和二-2-甲基-2-戊烯酸酯,收率分别为63.64%、65.23%和71.25%。并用它们对红豆杉植物细胞进行了染色研究,发现荧光素二-2-丁烯酸酯的生物荧光活性要好于荧光素的二丙烯酸及二-2-甲基-2-戊烯酸酯。
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| 关 键 词： | 荧光素酯 红豆杉细胞 染色 |
| 文章编号： | 1003-5214(2005)06-0440-04 |
Synthesis of Fluorescein Esters and Application of Them in Dyeing of Yew Cell |
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| YAN Xi-Long,WANG Wen-feng,ZHANG Su-feng,LI Yang,BAI Guo-yi,CHEN Li-gong. Synthesis of Fluorescein Esters and Application of Them in Dyeing of Yew Cell[J]. Fine Chemicals, 2005, 22(6): 440-443 |
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| Authors: | YAN Xi-Long WANG Wen-feng ZHANG Su-feng LI Yang BAI Guo-yi CHEN Li-gong |
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| Affiliation: | YAN Xi-long1,WANG Wen-feng2,ZHANG Su-feng1,LI Yang1,BAI Guo-yi1,CHEN Li-gong~1* |
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| Abstract: | Fluorescein diacrylate,di-2-butenoate and di-2-methyl-2-pentenoate were prepared from fluorescein and acrylic acid,2-butenoic acid and 2-methyl-2-pentenoic acid in 63.64%,65.23% and 71.25% yield respectively.They were applied in dyeing yew cells.The results show that the biological fluorescent activity of fluorescein di-2-butenoate is better than those of diacrylate and di-2-methyl-2-pentenoate. |
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| Keywords: | fluorescein esters yew cell dyeing |
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