| 小肠肠炎耶尔森菌天然抑菌剂的快速筛选 |
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| 引用本文： | 陈默,胡长鹰,王志伟,吴希阳,魏华琳. 小肠肠炎耶尔森菌天然抑菌剂的快速筛选[J]. 食品工业科技, 2010, 0(1) |
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| 作者姓名： | 陈默 胡长鹰 王志伟 吴希阳 魏华琳 |
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| 作者单位： | 1. 江南大学包装工程系,江苏无锡,214122;暨南大学包装工程研究所,广东珠海,519070
2. 暨南大学食品系,广东广州,510632 |
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| 基金项目： | 广东省自然科学基金项目(07005952); 珠海市科技计划项目(PB20051013) |
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| 摘 要： | 在96孔板中培养后利用酶标仪测定了天然酚类物质香兰素、异丁香酚、丁香酚、麝香草酚、愈创木酚存在下小肠肠炎耶尔森菌的光密度(OD)值,并绘制了相应的OD-t生长曲线,该测定方法具有快速、高通量、准确的优点。受试菌生长曲线表明:一定浓度的天然酚类物质对受试菌种有不同程度的抑制作用,其大小顺序为:异丁香酚>麝香草酚>丁香酚>香兰素>愈创木酚,对受试菌的最低抑菌浓度分别为660、1180、1280、1380、2040mg/L。
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| 关 键 词： | 小肠肠炎耶尔森菌 天然酚类物质 抗菌活性 生长曲线 快速筛选 |
Rapid screening of antimicrobial activity of natural phenolic compounds on Yersinia |
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| CHEN Mo,HU Chang-ying,WANG Zhi-wei,WU Xi-yang,WEI Hua-lin. Rapid screening of antimicrobial activity of natural phenolic compounds on Yersinia[J]. Science and Technology of Food Industry, 2010, 0(1) |
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| Authors: | CHEN Mo HU Chang-ying WANG Zhi-wei WU Xi-yang WEI Hua-lin |
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| Affiliation: | CHEN Mo1,3,HU Chang-ying2,WANG Zhi-wei1,WU Xi-yang2,WEI Hua-lin2(1.Department of Packaging Engineering,Jiangnan University,Wuxi 214122,China,2.Department of Food Science , Engineering,Jinan University,Guangzhou 510632,3.Packaging Engineering Institute,Zhuhai College,Zhuhai 519070,China) |
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| Abstract: | The Microplate Reader was used to detect the antimicrobial activity of vanilla,eugenol,isoeugenol,thymol and guaiacol against Yersinia enterocolitica(Y.enterocolitica)in a sterile 96-well microtitre plate.The growth curves of tested bacteria indicated that extracts and natural products exhibits certain antimicrobial effect against Y.enterocolitica,with minimum inhibitory concentration(MIC)values of 660,1180,1280,1380,2040mg/L,respectively.This method was proved to be economical,rapid and effective for detec... |
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| Keywords: | Yersinia enterocolitica natural phenolic products antimicrobial activity growth curve rapid screening |
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