Detection of herpes simplex virus in clinical specimens by cytospin-enhanced direct immunofluorescence |
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Authors: | ML Landry D Ferguson J Wlochowski |
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Affiliation: | Clinical Virology Laboratory, Yale New Haven Hospital, Connecticut, USA. |
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Abstract: | For 275 samples tested for herpes simplex virus (HSV), cytospin-enhanced direct immunofluorescence using Chemicon HSV monoclonal antibodies identified 80 (95%) and culture identified 77 (92%) of 84 confirmed positive specimens. Cytospin-prepared slides contained a greater number of total cells than standard cell spots, resulting in fewer inadequate cell smears and a higher HSV detection rate. |
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