Preparation of Biomolecule Microstructures and Microarrays by Thiol–ene Photoimmobilization |
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| Authors: | Dirk Weinrich Maja Köhn Dr. Pascal Jonkheijm Dr. Ulrika Westerlind Dr. Leif Dehmelt Dr. Hans Engelkamp Dr. Peter C. M. Christianen Dr. Jan C. Maan Prof. Dr. Dirk Nüsse Dr. Hendrik Schröder Dr. Ron Wacker Dr. Rolf Breinbauer Prof. Dr. Horst Kunz Prof. Dr. Christof M. Niemeyer Prof. Dr. Herbert Waldmann Prof. Dr. |
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| Affiliation: | 1. Department of Chemical Biology, Max Planck Institute of Molecular Physiology, Otto‐Hahn‐Strasse 11, 44227 Dortmund (Germany), Fax: (+49)?231‐133‐2499;2. Fakult?t Chemie, Chemische Biologie, Technische Universit?t Dortmund, Otto‐Hahn‐Strasse 6, 44227 Dortmund (Germany);3. Genome Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg (Germany);4. Molecular Nanofabrication, MESA+ Institute for Nanotechnology, University of Twente, P.?O. Box 217, 7500?AE Enschede (Netherlands);5. Department of Systems Biology, Max Planck Institute of Molecular Physiology, Otto Hahn Strasse 11, 44227 Dortmund (Germany);6. Institute for Molecules and Materials, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen (Netherlands);7. Fakult?t Elektrotechnik und Informationstechnik, Hochfrequenztechnik, Technische Universit?t Dortmund, Friedrich‐W?hler‐Weg 4, 44227 Dortmund (Germany);8. Fakult?t Chemie, Biologisch‐Chemische Mikrostrukturtechnik, Technische Universit?t Dortmund, Otto‐Hahn‐Strasse 6, 44227 Dortmund (Germany), Fax: (+49)231‐755‐7082;9. Institut für Organische Chemie, Technische Universit?t Graz, Stremayrgasse 16, 8010 Graz (Austria);10. Institut für Organische Chemie, Johannes‐Gutenberg‐Universit?t Mainz, Duesbergweg 10–14, 55128 Mainz (Germany) |
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| Abstract: | A mild, fast and flexible method for photoimmobilization of biomolecules based on the light‐initiated thiol–ene reaction has been developed. After investigation and optimization of various surface materials, surface chemistries and reaction parameters, microstructures and microarrays of biotin, oligonucleotides, peptides, and MUC1 tandem repeat glycopeptides were prepared with this photoimmobilization method. Furthermore, MUC1 tandem repeat glycopeptide microarrays were successfully used to probe antibodies in mouse serum obtained from vaccinated mice. Dimensions of biomolecule microstructures were shown to be freely controllable through photolithographic techniques, and features down to 5 μm in size covering an area of up to 75×25 mm were created. Use of a confocal laser microscope with a UV laser as UV‐light source enabled further reduction of biotin feature size opening access to nanostructured biochips. |
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| Keywords: | biochips immobilization microarrays photochemistry thiol– ene reaction |
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