| 超高效液相色谱-串联质谱法测定动物源性食品中的金刚烷胺 |
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| 引用本文： | 仲伶俐,郭灵安,付成平,李曦,雷欣宇,赵姗,李华仙.超高效液相色谱-串联质谱法测定动物源性食品中的金刚烷胺[J].食品安全质量检测技术,2018,9(6):1321-1325. |
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| 作者姓名： | 仲伶俐 郭灵安 付成平 李曦 雷欣宇 赵姗 李华仙 |
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| 作者单位： | 四川省农业科学院分析测试中心 |
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| 摘 要： | 目的建立超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)测定动物源性食品(鸡肉、鸡肝、鸡蛋和牛奶)中金刚烷胺的分析方法。方法样品用0.2%(V:V)甲酸乙腈溶液提取,经Oasis PRiME HLB小柱净化后用Capcell Pak C18 MGIII-H色谱柱分离,以甲醇和0.1%甲酸水溶液(V:V)作为流动相进行梯度洗脱,在多反应监测(multiple reaction monitoring,MRM)模式下进行液相色谱-串联质谱法测定,基质匹配标准溶液外标法定量。结果金刚烷胺在1.0~50.0μg/L范围内线性关系良好(r~2=0.9994)。在添加水平为1.0,5.0和20.0μg/kg时的回收率为76.3%~93.5%,相对标准偏差(relative standard deviation,RSD)在3.4%~8.6%之间,方法的检测限(limit of detection,LOD)为0.2μg/kg,定量限(limit of quantification,LOQ)为1.0μg/kg。结论该法简便、灵敏、准确,适合于动物源性食品中金刚烷胺的快速分析测定。
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| 关 键 词： | 超高效液相色谱-串联质谱法 金刚烷胺 动物源性食品 |
| 收稿时间： | 2017/12/5 0:00:00 |
| 修稿时间： | 2017/12/27 0:00:00 |
Determination of amantadine in animal derived food by ultra performance liquid chromatography-tandem mass spectrometry |
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| ZHONG Ling-Li,GUO Ling-An,FU Cheng-Ping,LI Xi,LEI Xin-Yu,ZHAO Shan and LI Hua-Xian.Determination of amantadine in animal derived food by ultra performance liquid chromatography-tandem mass spectrometry[J].Food Safety and Quality Detection Technology,2018,9(6):1321-1325. |
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| Authors: | ZHONG Ling-Li GUO Ling-An FU Cheng-Ping LI Xi LEI Xin-Yu ZHAO Shan and LI Hua-Xian |
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| Affiliation: | Analysis and Testing Center of Sichuan Academy of Agricultural Sciences,Analysis and Testing Center of Sichuan Academy of Agricultural Sciences,Analysis and Testing Center of Sichuan Academy of Agricultural Sciences,Analysis and Testing Center of Sichuan Academy of Agricultural Sciences,Analysis and Testing Center of Sichuan Academy of Agricultural Sciences,Analysis and Testing Center of Sichuan Academy of Agricultural Sciences and Analysis and Testing Center of Sichuan Academy of Agricultural Sciences |
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| Abstract: | |
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| Keywords: | ultra performance liquid chromatography-tandem mass spectrometry amantadine animal derived food |
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