基于MiSeq高通量测序技术内蒙古地区酸粥细菌多样性研究

从内蒙古地区采集了3个酸粥样品,使用MiSeq高通量测序技术,对其细菌多样性进行了评价。结果表明,厚壁菌门(Firmicutes)和变形菌门(Proteobacteria)为酸粥样品中的优势细菌门,其平均相对含量分别为87.45%和11.84%。相对含量大于1.0%的属为乳酸杆菌(Lactobacillus)和醋酸杆菌(Acetobacter),其平均相对含量分别为87.23%和11.67%。在分类操作单元(Operational taxonomic units,OTU)水平上,发现13个核心OTUs,其中8个隶属于乳酸杆菌(Lactobacillus),5个隶属于醋酸杆菌(Acetobacter)。通过传统微生物学手段从3个样品中共分离到9株乳酸菌,经鉴定为发酵乳杆菌(Lactobacillus fermentum)、植物乳杆菌(Lactobacillus plantarum)和卷曲乳杆菌(Lactobacillus curvatus)。由此可见,该地区酸粥中的细菌主要为乳酸杆菌(Lactobacillus)和醋酸杆菌(Acetobacter)。

Diversity of Bacteria Microflora in Acidic-Gruel of Inner Mongolia Area by MiSeq High Throughput Sequencing

The diversity of bacteria microflora of 3 bacterial microbiota samples of acidic-gruel collected from Inner Mongolia were studied by MiSeq high throughput sequencing technique. The results indicated that Firmicutes and Proteobacteria were the dominant bacterial phyla in acidic-gruel samples, with the average relative abundance of 87.45% and 11.84%. At the genera level, relatively abundant more than 1.0% of total bacteria in this population included Lactobacillus and Acetobacter, with the average relative abundance of 87.23% and 11.67%. At the OTU level, 13 OTUs were shared by all samples, and 8 OTUs were identified as Lactobacillus and 5 OTUs were identified as Acetobacter. In total, 9 isolates were obtained from 3 samples by conventional microbiological methods, and all these strains belonged to Lactobacillus fermentum, Lactobacillus plantarum and Lactobacillus curvatus. Thus, the domain bacteriums of acidic-gruel collected from Inner Mongolia were Lactobacillus and Acetobacter.