| 快速提取肉类基因组DNA的裂解液的研究 |
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| 引用本文： | 李婷婷,张桂兰,王之莹,陈爱亮. 快速提取肉类基因组DNA的裂解液的研究[J]. 食品安全质量检测学报, 2018, 9(17): 4755-4758 |
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| 作者姓名： | 李婷婷 张桂兰 王之莹 陈爱亮 |
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| 作者单位： | 中国农业科学院农业质量标准与检测技术研究所 |
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| 基金项目： | 国家重点研究与发展计划(2017YFF0211302) |
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| 摘 要： | 目的配制一种快速提取肉类DNA且满足重组酶聚合酶扩增技术(recombinase polymerase amplification,RPA)要求的裂解液。方法分别配制3种裂解液:Na OH裂解液、Chelex裂解液与PBS裂解液,取200μL裂解液与20 mg肉类样品进行混合,振荡5 s,取上清液稀释10倍直接作为扩增模板。结果 3种裂解液中Na OH裂解液提取DNA在稀释与未稀释条件下均有较好的扩增,Chelex裂解液提取DNA在稀释与未稀释下均无扩增,PBS裂解液提取DNA在稀释作用下有较好的扩增,在未稀释作用下无扩增。裂解液提取法和试剂盒方法提取的纯度、产物的大小几乎一致,所扩增的为同一产物,裂解液所需成本与试剂盒方法相比较低。结论 Na OH裂解液可以在5 min内提取肉类中的DNA,浓度与纯度均较高,所需时间短,成本低,且满足分子检测要求。
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| 关 键 词： | 重组酶聚合酶扩增技术 现场检测 肉类 裂解液 基因组DNA |
| 收稿时间： | 2018-05-22 |
| 修稿时间： | 2018-09-03 |
Study on rapid extraction lysate of meat genomic DNA |
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| LI Ting-Ting,ZHANG Gui-Lan,WANG Zhi-Ying and CHEN Ai-Liang. Study on rapid extraction lysate of meat genomic DNA[J]. Journal of Food Safety & Quality, 2018, 9(17): 4755-4758 |
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| Authors: | LI Ting-Ting ZHANG Gui-Lan WANG Zhi-Ying CHEN Ai-Liang |
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| Affiliation: | Institute of Quality Standards and Testing Technology for Agro-products,Chinese Academy of Agricultural Sciences,Institute of Quality Standards and Testing Technology for Agro-products,Chinese Academy of Agricultural Sciences,Institute of Quality Standards and Testing Technology for Agro-products,Chinese Academy of Agricultural Sciences and Institute of Quality Standards and Testing Technology for Agro-products,Chinese Academy of Agricultural Sciences |
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| Abstract: | |
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| Keywords: | recombinase polymerase amplification on-site detection meat lysate genomic DNA |
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