生牛肉中沙门菌的血清型鉴定与耐药性检测

目的了解新疆地区生牛肉中沙门菌的血清型和耐药状况。方法采用沙门菌诊断血清试剂盒检测血清型,微量肉汤稀释法检测分离菌株的耐药性。结果 28株牛肉源沙门菌共检出11种血清型,其5种优势血清型分别为伦敦沙门菌(17.9%)、德尔卑沙门菌(10.7%)、伤寒沙门氏菌(10.7%)、甲型副伤寒沙门菌(10.7%)和阿贡纳沙门菌(10.7%);沙门菌对10种抗菌药物耐药结果显示菌株对甲氧苄啶、氯霉素、四环素的耐药率最高,分别为100%、92.9%和75%,对氟苯尼考最为敏感,耐药率为7.1%。所有的菌株可耐2种或2种以上的抗生素。结论新疆地区牛肉源沙门菌存在一定的致病性,耐药状况比较严重。

Serotype identification and drug resistance detection of Salmonella isolated from raw beef

Objective To investigate the serotype and drug resistance of Salmonella isolated from raw beef in Xinjiang. Methods The serotype was detected by the Salmonella assay kit, and the drug resistance of the isolated strain was detected by the broth dilution method. Results The 28 strains of Salmonella were isolated from raw beef samples, and 11 kinds of serotypes were identified. The 5 dominant serotypes were S.london (17.9%), S. derby (10.7%), S. typhi (10.7%), S. paratyphia (10.7%) and S.agona (10.7%), respectively. The antimicrobial susceptibility of Salmonella to 10 kinds drugs was showed as follows: the highest drug resistance to trimethoprim (100%), followed by chloramphenicol (92.5%), tetracycline (75%); the lowest drug resistance to florfenicol (7.1%). All the strains were resistant to 2 or more antibiotics. Conclusion There is a certain pathogenicity for Salmonella isolated from raw beef in Xinjiang, and the drug resistance is serious.