| 高效液相色谱法检测牛奶及其制品中的溶菌酶 |
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| 引用本文： | 葛淑丽,戴玉婷,赵琴,王莹. 高效液相色谱法检测牛奶及其制品中的溶菌酶[J]. 食品安全质量检测学报, 2018, 9(17): 4628-4632 |
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| 作者姓名： | 葛淑丽 戴玉婷 赵琴 王莹 |
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| 作者单位： | 上海市质量监督检验技术研究院 |
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| 摘 要： | 目的建立一种测定牛奶及奶酪制品中低含量溶菌酶的高效液相色谱-荧光检测新方法。方法样品经p H 6.0的氯化钠溶液活化,再在低p H值条件下除蛋白后,采用反相色谱柱(PLRP-S 250 mm×4.6 mm,300?,5μm)用A水(0.1%的三氟乙酸(trifluoroacetic acid,TFA)),B乙腈(0.1%TFA)体系作为流动相进行梯度洗脱,使用荧光检测器在激发波长(λ_(ex))276 nm,发射波长(λ_(em))345 nm处检测。结果最优实验条件下,溶菌酶在2.0~30.0 mg/L浓度范围内线性良好,加标实验结果显示回收率和相对标准偏差(relative standard deviation,RSD)分别在92.3%~104.3%和0.81%~3.26%之间,对牛奶和奶酪制品的检出限分别为20和40 mg/kg。结论该方法操作简便,结果准确、可靠,可用于乳制品中溶菌酶含量测定。
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| 关 键 词： | 溶菌酶 高效液相色谱法 荧光检测 牛奶及奶酪制品 |
| 收稿时间： | 2018-07-03 |
| 修稿时间： | 2018-08-24 |
Determination of lysozyme in milk and its dairy products by high performance liquid chromatography |
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| GE Shu-Li,DAI Yu-Ting,ZHAO Qin and WANG Ying. Determination of lysozyme in milk and its dairy products by high performance liquid chromatography[J]. Journal of Food Safety & Quality, 2018, 9(17): 4628-4632 |
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| Authors: | GE Shu-Li DAI Yu-Ting ZHAO Qin WANG Ying |
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| Affiliation: | Shanghai Institute Of Quality Inspection and Technical Research,Shanghai,Shanghai Institute Of Quality Inspection and Technical Research,Shanghai,Shanghai Institute Of Quality Inspection and Technical Research,Shanghai and Shanghai Institute Of Quality Inspection and Technical Research,Shanghai |
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| Abstract: | |
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| Keywords: | lysozyme high performance liquid chromatography fluorescence detection milk and cheese |
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