培养基成分对酸土脂环酸芽孢杆菌生长及芽孢形成的影响

本研究通过单因素和双因素交互实验,研究多种营养因子对酸土脂环酸芽孢杆菌生长和芽孢形成的影响。结果表明,葡萄糖、酵母浸粉、Mn²⁺、Mg²⁺对酸土脂环酸芽孢杆菌的菌体生长和芽孢形成影响显著(p<0.05)。其中高浓度的Mn²⁺对酸土脂环酸芽孢杆菌的菌体生长有抑制作用,但能促进其芽孢形成。而Ca²⁺、NH₄⁺和K⁺单一作用时,对其菌体生长和芽孢形成影响不显著(p>0.05),但Ca²⁺与K⁺或者NH₄⁺协同作用时,可以促进酸土脂环酸芽孢杆菌的菌体生长和芽孢形成。研究结果揭示了培养基中的营养成分对该菌菌体生长和芽孢形成的影响,为控制该菌芽孢污染及酸性果蔬产品保藏提供实验和理论依据。

Effect of Medium Component on Growth and Spore Formation of Alicyclobacillus acidoterrestris

The effect of nutrient content on cell growth and spore formation of A. acidoterrestris DSM 3922T was investigated through single factor and multiple factors experiment. The results showed that glucose, yeast powder, Mn2+ and Mg2+ had significant effect on the cell density and the sporulation rate (p<0.05), among which high concentration of Mn2+ inhibited the growth of A. acidoterrestris, but promoted the formation of spores. And Ca2+, NH₄+ and K+ had a non-significant effect as a single factor on the cell density and the sporulation rate (p>0.05). However, the cooperation of Ca2+ with K+ or NH₄+ could promote cell growth and spore formation of A. acidoterrestris. The results revealed the influence of trophic factor on growth and spore formation of A. acidoterrestris, which would establish an experimental and theoretical foundation for the control of A. acidoterrestris spore contamination and the preservation of acidic fruit and vegetable products.