响应面优化超声波辅助乙醇提取橄榄叶总黄酮的工艺研究

为增加橄榄叶总黄酮和橄榄叶资源的开发利用价值,在超声波功率、乙醇浓度、超声时间、提取温度和料液比5个单因素实验基础上,以总黄酮得率为指标,应用响应面分析法对橄榄叶总黄酮的超声波辅助乙醇溶液提取工艺进行优化。结果表明,最佳工艺为超声波功率285 W,乙醇浓度80%,超声时间40 min,提取温度51 ℃,液料比25:1 mL/g。该工艺下橄榄叶总黄酮的得率达5.83％,与模型预测得率的相对误差只有2.64%。该提取工艺简单、高效,为工业化开发橄榄叶总黄酮,深度开发与充分利用橄榄叶资源提供了重要的参考。

Ultrasonic-assisted ethanol extraction techniques of the total flavonoids from the leaves of Canarium album by response surface methodology

The technology for ultrasonic-assisted extraction of total flavonoids from the leaves of Canarium album was optimized by response surface methodology in order to increase development and utilization value of the total flavonoids and the leaves of C. album. On the basis of the 5 single factor experiments. The results showed that the optimum technique were as follows:Ultrasonic power 285 W, ethanol concentration 80%, extraction time 40 min, extracting temperature 51℃ and liquid to solid 25:1 (mL/g). Under the above conditions, the total flavonoids yield of the leaves of C. album was 5.83%, which had 2.64% of the relative error to the model predictive yield. The optimum technology was simple and efficient, there was an important theoretical reference for industrial exploitation of the total flavonoids and widely exploitation and utilization of the leaves of C. album resource.