沙门氏菌及副溶血性弧菌的共增菌培养基的研制

为研制一种可同时富集沙门氏菌和副溶血性弧菌的增菌培养基,参照GB 4789.4-2016及GB 4789.7-2013规定的增菌培养基成分,并根据目标菌不同的营养需求,筛选适宜抑制剂和促进剂,进行单因素实验,确定共增菌培养基的配方,并验证该培养基的增菌效果。结果表明:研制出用于沙门氏菌和副溶血性弧菌的共增菌培养基成分为:蛋白胨10.0 g,磷酸二氢钾1.5 g,氯化钠7.5 g,硫代硫酸钠5.0 g,牛胆盐0.1 g,柠檬酸钠2.5 g,甘露醇2.5 g,葡萄糖2.5 g,蒸馏水1000 mL。目标菌初始接种量为102 CFU/mL,在37 ℃下培养16 h后,两种目标菌的菌浓度可达到107~108 CFU/mL。结果表明,共增菌培养基可用于沙门氏菌和副溶血性弧菌的富集培养,培养基配制简易,节约成本,具有良好的市场前景。

Preparation of Salmonella and Vibrio parahaemolyticus Co-enriched Culture Medium

To prepare a culture medium which could simultaneously increase Salmonella and Vibrio parahaemolyticus, the components of specific culture medium was based on GB 4789.4-2016 and GB 4789.7-2013, different nutritional requirements of the target bacteria were taken as references to select suitable accelerators and inhibitors. The culture medium components were determined and the enriching effects were verified in this study. Results showed that, the components of Salmonella and Vibrio parahaemolyticus co-enriched culture medium as follows:Peptone 10.0 g, potassium dihydrogen phosphate 1.5 g, sodium chloride 7.5 g, sodium thiosulfate 5.0 g, cattle bile salt 0.1 g, sodium citrate 2.5 g, mannitol 2.5 g, glucose 2.5 g, distilled water 1000 mL. The initial inoculum concentration of the target bacteria both were 102 CFU/mL, and the concentration could reach to 107~108 CFU/mL after culturing at 37℃ for 16 h. The results showed that the co-enriched culture medium could be used for the enrichment of Salmonella and Vibrio parahaemolyticus. The culture medium could be used for the enrichment and cultivation of Salmonella and Vibrio parahaemolyticus, which was easy to be prepared, cost-effective, and had a good market prospect.