植物乳杆菌C88对高脂饲料和链脲佐菌素诱导2型糖尿病模型大鼠的降血糖作用

目的：探究植物乳杆菌（Lactobacillus plantarum）C88对2型糖尿病大鼠模型降血糖作用。方法：采用高 脂饲料结合腹腔注射链脲佐菌素的方法建立2型糖尿病大鼠模型，连续灌胃植物乳杆菌C88，28 d后检测大鼠空腹血 糖量、葡萄糖耐受量、血脂、胰岛素、炎症因子、肠道屏障功能和菌群水平。Western blot分析核因子-κB（nuclear factor-κB，NF-κB）、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、闭锁小带蛋白-1（zonula occludens-1， ZO-1）、occludin、claudin-1的表达。结果：植物乳杆菌C88可有效改善2型糖尿病大鼠高血糖和血脂代谢紊乱；显 著降低2型糖尿病大鼠血清中TNF-α、白细胞介素-6、C反应蛋白、脂多糖、D-乳酸等炎症相关因子水平；植物乳杆 菌C88能促进胰岛素的分泌，提高胰高血糖素样肽-2水平，降低2型糖尿病大鼠血清中二胺氧化酶和连蛋白水平；显 著降低肝脏中炎症相关蛋白NF-κB、TNF-α的表达，提高回肠中肠道通透性相关蛋白ZO-1、occludin、claudin-1的表 达。结论：植物乳杆菌C88是一株具有辅助降血糖功能的益生菌。

Antidiabetic Effect of Lactobacillus plantarum C88 in a Rat Model of High-Fat Diet and Streptozotocin-Induced Type 2 Diabetes

Objective: To investigate the antidiabetic effect of Lactobacillus plantarum C88 in a type 2 diabetic rat model. Methods: Type 2 diabetes was induced in rats by feeding high-fat diet followed by single intraperitoneal injection of streptozotocin. The diabetic rats were intragastrically administrated with L. plantarum C88 for 28 consecutive days, and then the fasting blood-glucose, glucose tolerance, blood lipids, insulin, inflammatory factors, intestinal barrier function and intestinal flora were measured. Western blot was used to analyze the expression of nuclear factor-κB (NF-κB), tumor necrosis factor-α (TNF-α), zonula occludens-1 (ZO-1), occludin and claudin-1. Results: L. plantarum C88 could effectively improve hyperglycemia and lipid metabolism disorder in type 2 diabetic rats and significantly decrease the levels of TNF-α, interleukine-6, C-reactive protein, lipopolysaccharide, D-lactic acid and other inflammatory factors in the serum. L. plantarum C88 could promote the secretion of insulin, increase glucagon-like peptide-2 level and decrease diamine oxidase and zonulin. In addition, this strain significantly down-regulated the expression of inflammation-associated protein (NF-κB and TNF-α) in the liver and up-regulated the expression of ZO-1, occludin and claudin-1, associated with intestinal permeability in the ileum. Conclusion: L. plantarum C88 is a probiotic with hypoglycemic activity.