全自动葡萄酒成分分析仪测定葡萄酒中柠檬酸

目的建立全自动葡萄酒成分分析仪测定葡萄酒中柠檬酸的方法。方法仪器自动完成校准曲线的绘制、样品与试剂的混合、孵育、反应及340 nm波长处的比色检测。结果在0～0.5 g/L标准溶液浓度范围内,体系吸光度(Y)与溶液浓度(X)良好的函数关系,二阶拟合校准方程为:Y=0.6232X~2-0.6804X-0.00896,相关系数为0.9999,样品的加标回收率为94%～104%,同时与GB/T 15038-2006《葡萄酒、果酒通用分析方法》的高效液相色谱法进行了对比,相对标准偏差小于3%。结论该法灵敏度高、测定速度快、试剂使用量少,适用于不同类型葡萄酒中柠檬酸的大批量分析。

Determination of citric acid in wine by gallery automatic discrete analyzer

Objective To establish a method for determination of citric acid in wine by thermo scientific gallery automatic discrete analyzer. Methods Some steps including calibration curve drawing, sample and reagents mixing and incubation, and colorimetric detection of 340 nm wave length were realized automatically by this instrument. Results In the concentration range of 0~0.5 g/L, the absorbance (Y) was well related to the concentration (X), the second-order fitting calibration equation was Y=0.6232X2?0.6804X?0.00896, and the correlation coefficient was 0.9999. The standard recovery rates were 94%~104%. Meanwhile, compared with high performance liquid chromatography method from the national standard of China (GB/T 15038-2006 General analysis method for wine and fruit wine), the relative standard deviation was less than 3%. Conclusion This method has the advantages of high sensitivity, rapid determination speed and less reagent dosage, which can be used for the mass analysis of citric acid in different types of wines.